Arabidopsis VIP6/ELF8, the homolog of CTR9 component of the transcriptional complex PAF1, is essential for plant development
<i>The VERNALIZATION INDEPENDENCE 6</i> (<i>VIP6</i>)/<i>EARLY FLOWERING 8</i> (<i>ELF8</i>) gene of <i>Arabidopsis thaliana</i> encodes a protein homologous to CYCLIN THREE REQUIRING 9 (CTR9) component of the yeast transcriptional complex, RNA polymerase II-associated factor 1 (PAF1). It has been demonstrated that mutation alleles in the <i>VIP6</i>/<i>ELF8</i> show early flowering phenotype, as well as other pleiotropic developmental defects. Here, we provide evidence that seeds with <i>vip6</i>/<i>elf8</i> homozygous mutations were rarely obtained in all three independent lines of T-DNA insertion. Although viable seeds with homozygous mutant alleles were rarely obtained, they showed pleiotropic phenotype like mutants of other PAF1-related genes, and were sterile. These results suggest that the <i>VIP6</i>/<i>ELF8</i> gene is essential for plant development.
- Plant biotechnology
Plant biotechnology 25(5), 447-455, 2008-12-01
Japanese Society for Plant Cell and Molecular Biology