Suppressive expression of the glutelin A1-collagen peptide fusion gene in hybrid rice lines with the mutant line, Low Glutelin Content-1 (LGC-1)

• Hashizume Fujio

  Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University Mie Prefecture Agricultural Research Institute

• Hino Shingo

  Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University

• Okajima Tetsuya

  Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University

• Nadano Daita

  Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University

• Aoki Naohito

  Department of Life Science, Graduate School of Bioresources, Mie University

• Matsuda Tsukasa

  Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University

抄録

A low glutelin-content mutant, LGC-1, has a mutant gene (lgc1) comprising a tail-to-tail inverted repeat of two highly homologous glutelin B genes (gluB4 and δ-gluB5), resulting in the production of double-stranded glutelin B RNA. Hybrid rice lines between LGC-1 and a transgenic line with the glutelin A1-collagen peptide fusion gene (gluA1-coll) were produced, and F₂ hybrid lines homozygous for each or both of the gluA1-coll and lgc1 genes were selected. The expression of gluA1-coll in F₂ lines with lgc1 was far lower than that of F₂ lines without lgc1 at both the mRNA and protein levels as measured by semi-quantitative RT-PCR and immunoblotting using anti-collagen peptide antibody. The gluA1 mRNA sequence alignment identical to the potential double-stranded RNA formed by gluB4 sense and δ-gluB5 antisense RNAs consisted of 14 nucleotides at most, and was not more than 23 even when one mismatch was allowed within the sequence. Thus, such shorter double-stranded glutelin B RNAs were suggested to suppress the expression of not only endogenous gluA genes as reported previously (Kusaba et al. 2003) but also the exogenous gluA1-coll in the hybrid lines.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 25 (5), 465-471, 2008

  日本植物バイオテクノロジー学会

キーワード

• glutelin multigene family
• low glutelin-content mutant
• RNA interference
• transgenic rice
• type II-collagen

詳細情報

• CRID: 1390282679305185024
• NII論文ID: 10021914665
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.25.465
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 9654504
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9654504
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
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