Du3, a mRNA cap-binding protein gene, regulates amylose content in Japonica rice seeds

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Five non-allelic <i>dull</i> (<i>du</i>) mutants have been isolated and characterized as low amylose mutants in rice (<i>Oryza sativa</i>) endosperm. Synthesis of amylose is controlled by <i>Waxy</i> the gene that encodes granule-bound starch synthase. We previously showed that the <i>du1</i> and <i>du2</i> proteins may encode general splicing regulators which affect the expression level and splicing efficiency of <i>Waxy<sup>b</sup></i> (<i>Wx<sup>b</sup></i>), an allele that has a single-base mutation in the 5′ splice site of intron 1. Here we report that the <i>du</i>3 mutant has also reduced the splicing efficiency of <i>Wx<sup>b</sup></i> pre-mRNA. Furthermore, map-based cloning revealed that <i>Du3</i> encodes the rice homolog of a cap-binding protein 20 kD subunit (CBP20), a component of the heterodimeric nuclear cap-binding complex (CBC) playing a role in pre-mRNA splicing, RNA nuclear export, and nonsense-mediated decay. Transient expression of green fluorescent protein (GFP)-tagged <i>Du3</i> proteins in rice protoplasts resulted in localization to the nucleus showing a speckled pattern similar to the pattern displayed by other splicing factors.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 25(5), 483-487, 2008-12-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021914714
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    13424580
  • NDL 記事登録ID
    9654515
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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