Rapid and simple DNA extraction method from rice using a glass-fiber filter inserted pipette tip

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A novel and simple DNA extraction method was developed, which was called "the filter-inserted tip method". This method used glass fiber filters inserted within micropipette tips. It was conducted by drawing and expelling fluid by pipetting using a multichannel pipette. This allowed us to manipulate a large number of samples at the same time for DNA adsorption, fixing, washing and extraction. The 3 mm×3 mm size of glass fiber filter was suitable for this method. To examine the reproducibility of the method, DNA extraction experiment was carried out using rice leaves, and the yielded DNA samples were used for PCR reactions. As a result, PCR amplifications were recognized for 234 out of 236 samples examined, showing high reproducibility of the method. It took approximately 20 min to conduct DNA extractions from 16 samples. These results suggest that this method is suitable for rapid DNA extractions from a large number of samples for PCR reactions, such as marker-assisted selection with simple manipulations, low cost, and without using expensive equipment.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 25(5), 493-496, 2008-12-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021914769
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    9654532
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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