A stress-responsive multifunctional protein involved in β-oxidation in tobacco plants
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Biotic and abiotic-induced wounding is one of the severest stresses that plants suffer throughout the growing period. Upon injury, plants rapidly activates a set of genes, which encode diverse proteins to cope with damages. Screening such genes that were transcriptionally activated within 30 min after mechanical wounding in tobacco plants, we identified a particular clone encoding a multfunctional protein, and designated as NtMFP (<i><u>N</u>icotiana <u>t</u>abacum</i> <u>m</u>ulti<u>f</u>unctional <u>p</u>rotein). The deduced polypeptide is constituted of 668 amino acids with an apparent molecular mass of 72.3 kDa, and bacterially expressed protein exhibited a clear β-oxidation activity. Fusion proteins with GFP were observed in cytosol, when expressed in onion epidermal cell layers. In addition to wounding, <i>NtMFP</i> transcripts were induced by tobacco mosaic virus infection, and by jasmonic acid treatments. When <i>NtMFP</i> was suppressed by the RNAi, transgenic tobacco showed dwarfism, early senescence and reduced expresstion of jasmonic acid-responsive genes. Multifunctional protein is generally considered to catalyze multiple steps of the fatty acid β-oxidation. It is also proposed to be involved in the β-oxidation step of jasmonic acid biosynthesis. The present results suggest the possibility that NtMFP commonly functions not only in fatty acid catabolism but also in jasmonic acid biosynthesis pathway.
- Plant tissue culture letters
Plant tissue culture letters 25(5), 503-508, 2008-12-01
Japanese Society for Plant Cell and Molecular Biology