黄砂発生源におけるバイオエアロゾル拡散に関する研究 Study on Atmospheric Diffusion of Bioaerosols in a KOSA Source Region
In order to clarify the behavior of bioaerosols diffusing in the atmosphere with KOSA (yellow sand) , we attempted to identify the bioaerosols in the atmospheric mixed layer over the KOSA source region, Dunhuang, China. Bioaerosols were collected at 50-100 m (using a tethered balloon) and at 10 m above the ground, and microorganisms, as the source of bioaerosols, were collected from desert sand. The bioaerosols were cultivated immediately after the collection at the Dunhuang City meteorological station. 20 strains of isolates were grouped into three major categories according to the morphology and physiological characteristics: Gram-positive rod (15 strains) , Gram-negative rod (3 strains) , and Gram-positive cocci (2 strains) . The 500-bp 16S rDNA partial sequences of 7 strains isolated from the samples collected 50-100 and 10 m above the ground showed identity. Bioaerosols appear to rise in the atmospheric mixed layer from near the ground to the upper ground with KOSA. The results of a homology search by 16S rDNA or 18S rDNA sequences of isolates in DNA databases (GenBank, DDBJ, and EMBL) revealed that bioaerosols in the atmospheric mixed layer over the KOSA source region contain <I>Bacillus cereus</I> (50-100 and 10 m above the ground) , <I>Rhodosporidium sphaerocarpum</I> (50-100 m above the ground) , <I>Candida parapsilosis</I> (10 m above the ground) , <I>Pantoea agglomerans</I> (10 m above the ground) , and <I>Enterobacter endosymbiont</I> (10 m above the ground) and that microorganisms from the sand were <I>Bacillus subtilis</I> and <I>Bacillus atrophaeus</I>. Although <I>Bacillus cereus</I> isolated from the samples collected 50-100 and 10 m above the ground was a kind of food spoilage or pathogenic bacterium, our findings indicate that the KOSA bioaerosol diffusion may influence human and natural environments.
- エアロゾル研究 = Journal of aerosol research
エアロゾル研究 = Journal of aerosol research 22(3), 218-227, 2007-09-20