Identification of food-borne fungi using MicroPlate system, by the utilization of carbon sources

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  • ICHINOE Masakatsu
    Department of Nutrition, Faculty of Domestic Science, Tokyo Kasei University Department of Food and Nutrition, Graduate School, Tokyo Kasei University
  • UMEBARA Yuuka
    Department of Nutrition, Faculty of Domestic Science, Tokyo Kasei University
  • TAKIGAWA Midori
    Department of Nutrition, Faculty of Domestic Science, Tokyo Kasei University
  • MATSUMARU Keiko
    Department of Food and Nutrition, Graduate School, Tokyo Kasei University

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Other Title
  • 炭素源利用能データベースによる食品由来カビの識別・同定

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Abstract

During investigation of fungal flora in imported and domestic cereal products many isolates of Penicillium were obtained. Of the representative 20 Penicillium strains, the MicroLog system was tested for species identification. The MicroLog system is a 96 well microplate with 95 different carbon sources selected to include all of the important biochemical groups: sugers, carboxylic acids, amino acids, fattyacids, etc. The FF MicroPlate was used in this study and purchased from BIOLOG, Inc. One plate is inoculated with one isolate conidial suspension. After incubation, plates were evaluated with a microplate reader, which measures the turbidity in each well. The microplate reader connected to a computer, using the MicroLog software. During the incubation of the MicroPlates it was observed that most of the isolates produced colored soluble pigments on some of the carbon sources. Some species of the genus Penicillium are difficult to separate based on morphology alone, and the differentiation between species of this genus is difficult without the aid of physiological tests, i.e, assimilation of carbon sources and secondary metabolic profiles. We found that the MicroLog system could distinguish between the limited number of mycotoxigenic Penicillia such as P. citreonigrum, P. citrinum, P. expansum, P. islandicum and P. verrucosum. In addition, we examined to distinguish among the members of aflatoxigenic section Flavi species, including Aspergillus flavus, A. parasiticus and A. nomius isolates from imported peanuts and sugarcane field soil collected from Okinawa by the MicroLog system. Our results indicate that difficult of separate of these species.

Journal

  • JSM Mycotoxins

    JSM Mycotoxins 56 (2), 85-90, 2006

    Japanese Society of Mycotoxicology

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