アフラトキシンB<sub>1</sub> 誘導ラット肝癌由来K2 細胞は癌幹細胞の性質を有している

DOI 日本農学文献記事索引 被引用文献3件 参考文献33件

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  • Cancer stem cells in aflatoxin B1-induced rat hepatocellular carcinoma K2 cells

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In this study, we performed the side population (SP) cell analysis and the expression analysis of stemness genes to identify stem-like cells (cancer stem cells) in aflatoxin B1-(AFB1) induced rat hepatoma K2 cells. Using the flow cytometric analysis with rhodamine 123, the SP cells, which are thought to be cancer stem cells, were detected with high frequency and estimated to be approximately 89 % of K2 cell population. Furthermore, K2 cells expressed stemness genes including the sox2, nanog, klf4, the ras family E-ras and the polycomb family bmi1 genes, which are implicated in the maintenance of pluripotency and self-renewal of embryonic stem (ES) cells. On the other hand, in K2 cells the expression levels of mature hepatocyte-specific marker genes such as albumin and tyrosine aminotransferase (TAT) were very low. Thus, these results imply that K2 cells possess the typical properties of cancer stem cells through the acquisition of stemness gene expressions.

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