Cancer stem cells in aflatoxin B1-induced rat hepatocellular carcinoma K2 cells

DOI JASI 3 Citations 33 References
  • KAWASAKI Yasushi
    Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
  • ADACHI Naomi
    Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
  • YAMAZAKI Tomio
    Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
  • TODOROKI Risa
    Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
  • GOTOU Yoshitaka
    Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
  • KOMIYA Yuko
    Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
  • KURABE Nobuya
    Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
  • NEMOTO Kiyomitsu
    Department of Molecular Toxicology, School of Pharmaceutical Sciences, University of Shizuoka
  • DEGAWA Masakuni
    Department of Molecular Toxicology, School of Pharmaceutical Sciences, University of Shizuoka
  • MIURA Shigetoshi
    Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
  • TASHIRO Fumio
    Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science

Bibliographic Information

Other Title
  • アフラトキシンB<sub>1</sub> 誘導ラット肝癌由来K2 細胞は癌幹細胞の性質を有している

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Abstract

In this study, we performed the side population (SP) cell analysis and the expression analysis of stemness genes to identify stem-like cells (cancer stem cells) in aflatoxin B1-(AFB1) induced rat hepatoma K2 cells. Using the flow cytometric analysis with rhodamine 123, the SP cells, which are thought to be cancer stem cells, were detected with high frequency and estimated to be approximately 89 % of K2 cell population. Furthermore, K2 cells expressed stemness genes including the sox2, nanog, klf4, the ras family E-ras and the polycomb family bmi1 genes, which are implicated in the maintenance of pluripotency and self-renewal of embryonic stem (ES) cells. On the other hand, in K2 cells the expression levels of mature hepatocyte-specific marker genes such as albumin and tyrosine aminotransferase (TAT) were very low. Thus, these results imply that K2 cells possess the typical properties of cancer stem cells through the acquisition of stemness gene expressions.

Journal

  • JSM Mycotoxins

    JSM Mycotoxins 57 (2), 87-93, 2007

    Japanese Society of Mycotoxicology

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