Identification of the attached chromosome in sex-limited normal marked silkworm, Bombyx mori.

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  • Ikejima Satomi
    Institute of Sericulture United Graduate School of Agricultual Science, Tokyo University of Agriculture and Technology
  • Tsuneyama Izumi
    Institute of Sericulture
  • Tanaka Yukio
    Institute of Sericulture
  • Ninagi Osamu
    United Graduate School of Agricultual Science, Tokyo University of Agriculture and Technology
  • Hara Wajiro
    National Institute of Agrobiological Sciences

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Other Title
  • カイコ限性形蚕系統における付着染色体の識別同定
  • カイコ ゲンセイケイ カイコ ケイトウ ニ オケル フチャク センショクタイ ノ シキベツ ドウテイ

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Abstract

Genomic Southern blot hybridization of two sex-limited silkworm strains, W3a and W73, which show the female specific larval markers, crescent mark and star spot, were carried out to identify the areas of attached second chromosome by using RFLP probes of the ESTcDNA clones. When the two clones, e61 and m113 on the second linkage group were used, W3a showed female specific bands. On the other hand, even if these clones were used, W73 showed no difference in the band pattern between females and males. When other clones on the second linkage group were used, both strains showed no difference in the band pattern between females and males. This result indicated that the area of e61-m113 was included in the attached chromosome of W3a. The difference of the area of the attached chromosome between these strains suggested that the length of the W3's attached chromosome was longer than W73's.<br>

Journal

  • Sanshi-Konchu Biotec

    Sanshi-Konchu Biotec 77 (2), 2_145-2_151, 2008

    The Japanese Society of Sericultural Science

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