高密度試料スポット技術のレーザー脱離イオン化質量分析への応用 Application of High-Density Sample Spotting to Laser Desorption/Ionization Mass Spectrometry

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著者

    • 青木 寛 AOKI Hiroshi
    • 独立行政法人産業技術総合研究所環境管理技術研究部門 Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST)
    • 佐藤 浩昭 SATO Hiroaki
    • 独立行政法人産業技術総合研究所環境管理技術研究部門 Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST)
    • 根本 淳史 [他] NEMOTO Atsushi
    • 独立行政法人産業技術総合研究所環境管理技術研究部門 Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST)
    • 田尾 博明 TAO Hiroaki
    • 独立行政法人産業技術総合研究所環境管理技術研究部門 Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST)

抄録

Novel aspects of laser desorption/ionization mass spectrometry were demonstrated using a recently developed 8-ch capillary-based dispensing workstation with a variable capillary pitch mechanism. Its application to improve mass accuracy in external mass calibration for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was investigated using angiotensin-I, as both of reference and sample compounds positioned close to each other on a target plate. The workstation's high-density dispensing was found to provide an 8×8 spot array, with a spot interval of 1 mm, and a standard deviation of mass errors of 0.02 (16 ppm) for 64 spots in the array. Thus, the mass accuracy was comparable to that normally observed in internal mass calibration. The workstation was also applied to dispensing small volumes of sample solutions of octylphenol polyethoxylate and α-cyclodextrin with high-density to substrates with self-assembled germanium nanodots (GeNDs) for surface-assisted laser desorption/ionization (SALDI). An 8×8 spot array, with a spot interval of 1 mm, was successfully prepared on the GeND substrates. Previously, this process required difficult preparation of multiple spots, either by dispensing them manually, or using conventional dispensers based on pipette tips.

収録刊行物

  • 質量分析 = Mass spectroscopy  

    質量分析 = Mass spectroscopy 56(5), 223-228, 2008-10-01 

    The Mass Spectrometry Society of Japan

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各種コード

  • NII論文ID(NAID)
    10021921540
  • NII書誌ID(NCID)
    AN0010555X
  • 本文言語コード
    JPN
  • 資料種別
    NOT
  • ISSN
    13408097
  • NDL 記事登録ID
    9675478
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z17-213
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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