赤外レーザーマトリックス支援レーザー脱離イオン化法の波長依存性に関する研究と応用 Wavelength Dependence of Infrared Matrix-Assisted Laser Desorption/Ionization and Its Application

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著者

    • 鈴木 幸子 SUZUKI Sachiko
    • 大阪大学大学院工学研究科環境・エネルギー工学専攻 Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University
    • 藤田 珠美 FUJITA Tamami
    • 大阪大学大学院工学研究科環境・エネルギー工学専攻 Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University
    • 粟津 邦男 AWAZU Kunio
    • 大阪大学大学院工学研究科環境・エネルギー工学専攻 Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University

抄録

Infrared matrix-assisted laser desorption/ionization (IR-MALDI) using an infrared laser is promising, because most biomolecules have a specific absorption in the infrared range. IR-MALDI is expected to provide the following advantages: (1) use of various matrices; (2) use of biomolecules such as water and lipids as the matrix; and (3) super-soft ionization, i.e., high-molecular-weight protein analysis without fragmentation. However, IR-MALDI is not widely used because of its low sensitivity, complexity, high cost, and low-compatibility with commercial MALDI time-of-flight mass spectrometers. We evaluated the wavelength dependence of ionization in IR-MALDI to clarify the ionization mechanism. The molecular mass of angiotensin II was obtained at a wavelength between 5.8 and 6.2 μm, which corresponds to the >C=O stretching vibration mode. In IR-MALDI, we believe that the strong molecular bond attracts an electron from a neighboring hydrogen atom, possibly protonating it.

収録刊行物

  • 質量分析 = Mass spectroscopy  

    質量分析 = Mass spectroscopy 56(5), 235-240, 2008-10-01 

    The Mass Spectrometry Society of Japan

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各種コード

  • NII論文ID(NAID)
    10021921571
  • NII書誌ID(NCID)
    AN0010555X
  • 本文言語コード
    JPN
  • 資料種別
    REV
  • ISSN
    13408097
  • NDL 記事登録ID
    9675516
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z17-213
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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