Sensitive and Rapid Detection of <I>Mycoplasma pneumoniae</I> by Loop-mediated Isothermal Amplification

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  • LAMP (Loop-Mediated Isothermal Amplification) 法による<I>Mycoplasma pneumoniae</I> の高感度迅速検出
  • LAMP(Loop-Mediated Isothermal Amplification)法によるMycoplasma pneumoniaeの高感度迅速検出
  • LAMP Loop Mediated Isothermal Amplification ホウ ニ ヨル Mycoplasma pneumoniae ノ コウカンド ジンソク ケンシュツ
  • Sensitive and Rapid Detection of Mycoplasma pneumoniae by Loop-mediated Isothermal Amplification

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Abstract

For establishment of a method for rapid diagnosis of Mycoplasma pneumoniae in clinical specimens, we designed and evaluated a loop-mediated isothermal amplification (LAMP) assay, using a set of primers targeting the SDC1 repetitive element of the M. pneumoniae genome.<BR>The method showed rapid and specific amplification for all the strains of M. pneumoniae tested (Type Iand II), within 1hour at 65°C.<BR>No cross-reactivity with the most common causative organisms bacterial pneumonia was observed.<BR>The detection limit was shown as 6 copies, which was equal to or higher than that of the two nested PCRs used as references.<BR>Two hundred four clinical samples, comprising sputum samples, throat swabs, etc., were tested by both LAMP and PCR, and determination of the correlations revealed complete agreement individually (24 posi-tives).<BR>The LAMP assay, a simple procedure in a closed system, allowed rapid amplification and accurate de-tection consistently; therefore we considered that it could become an caeasily available diagnostic methodsuitable for clinical situations requiring quick and appropriate decisions for treatments and care.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 82 (3), 168-176, 2008

    The Japanese Association for Infectious Diseases

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