Detection of <I>Chlamydia trachomatis</I> and <I>Neisseria gonorrhoeae</I> in Urine Samples of Males and Females by the Strand Displacement Amplification (SDA) Method

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  • KANAYAMA Akiko
    Infectious Disease Testing Department Mitsubishi Chemical Medience Corporation
  • FUJIHARA Eriko
    Infectious Disease Testing Department Mitsubishi Chemical Medience Corporation
  • SAIKA Takeshi
    Infectious Disease Testing Department Mitsubishi Chemical Medience Corporation
  • KOBAYASHI Intetsu
    Infectious Disease Testing Department Mitsubishi Chemical Medience Corporation
  • ONOYE Yasuhiko
    Miyamoto-cho Chuoh Clinic

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Other Title
  • 男女尿検体におけるStrand displacement amplification (SDA) 法を用いた<I>Chlamydia trachomatis</I> および<I>Neisseria gonorrhoeae</I>の検出
  • 男女尿検体におけるStrand displacement amplification(SDA)法を用いたChlamydia trachomatisおよびNeisseria gonorrhoeaeの検出
  • ダンジョ ニョウ ケンタイ ニ オケル Strand displacement amplification SDA ホウ オ モチイタ Chlamydia trachomatis オヨビ Neisseria gonorrhoeae ノ ケンシュツ
  • Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Urine Samples of Males and Females by the Strand Displacement Amplification (SDA) Method

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Abstract

Urine samples collected from 422 males and 53 females visiting a clinic in Kawasaki City who were sus-pected to have sexually transmitted infection were tested for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae by BD ProbeTecET (SDA method). The detection rates of C. trachomatis by the SDAmethod and polymerase chain reaction (PCR) method (control) were as high as 98.1 % for C. trachomatis, andas high as 99.4% for N. gonorrhoeae, and the concordance rate of detection of both bacterial species was high.The detection sensitivity and specificity of the SDA method were 90.6 and 99.3%, respectively for C. trachomatis and 98.7% and 100% for N. gonorrhoeae, when PCR was used as the standard method. There wereno differences in these results between males and females. The number of patients showing a discrepancyof the results obtained between the SDA method and the PCR method was 9 for C. trachomatis and 1 for N.gonorrhoeae, but the results of redetermination by the SDA method tended to coincide with those of the PCRmethod. Urine samples tested by the SDA method were positive for N. gonorrhoeae even in patients in whomthe culture of secretions from the male urethra was negative for N. gonorrhoeae.<BR>Based on these results, the BD Probe Tec ET (SDA method) was confirmed to have the equivalent capability to the PCR method for the detection of C. trachomatis and N. gonorrhoeae in urine samples.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 82 (3), 182-186, 2008

    The Japanese Association for Infectious Diseases

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