Early Diagnosis of Cancer by Detecting the Chemiluminescence of Hematoporphyrins in Peripheral Blood Lymphocytes
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- Kohno Masahiro
- New Industry Creation Hatchery Center, Tohoku University
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- Takeda Motohiro
- Division of Surgical Oncology, Graduate School of Medicine, Tohoku University
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- Niwano Yoshimi
- New Industry Creation Hatchery Center, Tohoku University
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- Saito Rumiko
- New Industry Creation Hatchery Center, Tohoku University
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- Emoto Natsumi
- New Industry Creation Hatchery Center, Tohoku University
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- Tada Mika
- New Industry Creation Hatchery Center, Tohoku University
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- Kanazawa Takemichi
- Medical Corporation Murakami Hospital
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- Ohuchi Noriaki
- Division of Surgical Oncology, Graduate School of Medicine, Tohoku University
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- Yamada Rie
- Tohoku Electronic Industrial Co., Ltd.
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抄録
Early detection and optimal treatment are the most effective means to improve cancer mortality. Mass screening for cancer has yielded a marked reduction of cancer mortality in the United States. Simple and effective methods are expected for screening of malignancy. Hematoporphyrin derivatives (HPDs) are known to accumulate in cancer cells; thus, HPD has been used for local diagnosis and photodynamic therapy of cancer. The lymphocytes of cancer patients also demonstrate the active uptake of HPD and this phenomenon has been applied for the diagnosis of cancer. In the present study, we have developed a novel method for measurement of the chemiluminescence of HPD in peripheral blood lymphocytes. HPD is composed of hematoporphyrin and its oligomers. Seven cancer patients and seven controls were recruited for this study. The primary cancers included two prostate cancers (one without metastasis and the other with lung metastasis), a renal cancer, a lung adenocarcinoma with systemic metastasis, two gallbladder cancers with lung metastasis, and a colon cancer with liver metastasis. HPD in lymphocytes was measured using a highly sensitive chemiluminescence analyzer with laser light irradiation to detect photoemission by 1O2 from HPD. The intensity of chemiluminescence exhibited a linear correlation with the concentrations of HPD. In addition, the level of HPD in lymphocytes was significantly higher in cancer patients than that in healthy volunteers (p < 0.05). These results suggest that detection of the chemiluminescence of HPD in lymphocytes could be a sensitive and simple method for cancer diagnosis and screening.
収録刊行物
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- The Tohoku Journal of Experimental Medicine
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The Tohoku Journal of Experimental Medicine 216 (1), 47-52, 2008
東北ジャーナル刊行会
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詳細情報 詳細情報について
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- CRID
- 1390282679218782080
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- NII論文ID
- 10021951618
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- NII書誌ID
- AA00863920
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- ISSN
- 13493329
- 00408727
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可