低濃度過酸化水素を用いた新規歯の漂白剤の検討―電子スピン共鳴（ＥＳＲ）法による活性酸素種検出技術の歯科臨床応用―

• 前谷 崇志

  神奈川歯科大学生体管理医学講座・薬理学分野・ESR研究室

• 吉野 文彦

  神奈川歯科大学生体管理医学講座・薬理学分野・ESR研究室

• 吉田 彩佳

  神奈川歯科大学生体管理医学講座・薬理学分野・ESR研究室

• 杉山 秀太

  神奈川歯科大学生体管理医学講座・薬理学分野・ESR研究室

• 西村 知子

  神奈川歯科大学口腔治療学講座・歯内療法学分野

• 石井 信之

  神奈川歯科大学口腔治療学講座・歯内療法学分野

• 李 昌一

  神奈川歯科大学生体管理医学講座・薬理学分野・ESR研究室

書誌事項

タイトル別名

• An investigation of application of novel tooth bleaching using low concentration of hydrogen peroxide-Dental application of electron spin resonance (ESR) technique for detecting reactive oxygen species-
• —Dental application of electron spin resonance (ESR) technique for detecting reactive oxygen species—
• ―電子スピン共鳴 (ESR) 法による活性酸素種検出技術の歯科臨床応用―

抄録

A high concentration of hydrogen peroxide (H₂O₂) or H₂O₂/titanium dioxide (TiO₂) is commonly used as a dental bleaching agent. The mechanism of tooth bleaching is that the reactive oxygen species (ROS), especially hydroxyl radical (HO^⋅), derived from H₂O₂ in the bleaching agent remove the color of teeth due to decomposition of organic substances. It has been reported that oxidative stress induced by ROS such as HO^⋅ could be involved in various oral diseases. Thus, H₂O₂ has been shown to be a risk factor for oral tissue injury during bleaching treatment. However, questions remain about the effects of ROS generated from bleaching treatment in detail. Electron spin resonance (ESR) is recognized as one of the most powerful techniques available for the detection of ROS in tissues and cells. We previously developed an ESR-based technique for the detection of ROS reactions in biological systems. In this study, we confirmed O₂^⋅- generated from a low concentration of H₂O₂ (0.3%) /TiO₂ with irradiation of UV light. It was observed that O₂^⋅- production from H₂O₂ (0.3%) /TiO₂ increased in a time-dependent manner of UV light irradiation. Both the L value and ΔE^*ab value rose in H₂O₂ (0.3%) /TiO₂ with irradiation of UV light. These results suggest that a low concentration H₂O₂/TiO₂ system could be applied more safely due to the formation O₂^⋅- but not HO^⋅ as a risk factor for bleaching. In addition, the ESR technique would be useful for characterizing ROS for dental bleaching agents.

収録刊行物

• 歯科薬物療法

  歯科薬物療法 27 (2), 109-115, 2008

  日本歯科薬物療法学会

キーワード

• 活性酸素種
• 二酸化チタン光触媒
• 電子スピン共鳴法
• 過酸化水素
• スーパーオキサイド
• ヒドロキシルラジカル
• 歯の漂白法

詳細情報

• CRID: 1390282679710282752
• NII論文ID: 130004040555
• NII論文ID: 10021954505
• NII書誌ID: AN0023517X
• DOI: 10.11263/jsotp1982.27.109
• ISSN: 18844928
• ISSN: 02881012
• Web Site: https://search.jamas.or.jp/link/ui/2008320192
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可