Surface Modification Enhances Osteoblast Behavior and Bone Formation on Thin Hydroxyapatite Layers Deposited Using a Novel Anodization-Hydrothermal Treatment on Commercially Pure Titanium Endosseous Implants

• Takebe Jun

  Department of Fixed Prosthodontics, School of Dentistry, Iwate Medical University

• Nakasato Yoshihiro

  Department of Fixed Prosthodontics, School of Dentistry, Iwate Medical University

• Ito Shigeki

  Department of Fixed Prosthodontics, School of Dentistry, Iwate Medical University

• Kikuchi Seiichiro

  Department of Fixed Prosthodontics, School of Dentistry, Iwate Medical University

• Itoh Sozo

  Department of Fixed Prosthodontics, School of Dentistry, Iwate Medical University

• Shioyama Tsukasa

  Department of Fixed Prosthodontics, School of Dentistry, Iwate Medical University

• Ishibashi Kanji

  Department of Fixed Prosthodontics, School of Dentistry, Iwate Medical University

Abstract

In the present study, we assessed the effects of commercially pure titanium (cpTi) by anodic oxidation and hydrothermal treatment (SA-treated cpTi) on osteoblastic differentiation and interfacial bone formation through parallel in vitro and in vivo investigations. Osteoblast cells were cultured on SA-treated cpTi disks for 5, 7, 10, and 14 days. Bone matrix mineralization was assessed by EPMA. The levels of collagen I, alkaline phosphatase, osteocalcin, osteopontin, bone sialoprotein, and β-actin mRNA were analyzed using RT-PCR. In addition, SA-treated cpTi implants were placed in the mandibles of beagles for 14 days, and then examined histologically by light microscopy. Widespread Ca and P signals were observed early in the in vitro culturing period, and mRNA expression was up-regulated in cells that were in contact with the SA-treated cpTi. The bone-to-implant contact formed at the mandible SA-treated cpTi implant sites involved direct contact of the implant with the surrounding bone tissue. These results demonstrate the potential of SA-treated cpTi surfaces for enhancing surface-specific expression of osteoblastic phenotypes and for inducing changes in bone matrix gene expression.

Journal

• Prosthodontic Research & Practice

  Prosthodontic Research & Practice 7 (2), 159-161, 2008

  Japan Prosthodontic Society

Keywords

• discharge anodic oxidation and hydrothermal treatment
• titanium
• endosseous implant
• osseointegration
• osteoblast differentiation

Details

• CRID: 1390001205195623424
• NII Article ID: 10021955128
• NII Book ID: AA11728086
• DOI: 10.2186/prp.7.159
• ISSN: 13477021
• Web Site: http://www.jstage.jst.go.jp/article/prp/7/2/7_2_159/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009105267
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed