Adhesion of C. albicans to Acrylic Resin Containing Fluoridated Apatite-Coated TiO2

• Sawada Tomofumi

  Division of Removal Prosthetics, Department of Oral and Maxillofacial Rehabilitation, Kanagawa Dental College

• Kimoto Katsuhiko

  Division of Fixed Prosthodontics, Department of Oral and Maxillofacial Rehabilitation, Kanagawa Dental College

• Takahashi Yusuke

  Division of Microbiology, Department of Infection Control, Kanagawa Dental College

• Sawada Tomoji

  Division of Removal Prosthetics, Department of Oral and Maxillofacial Rehabilitation, Kanagawa Dental College

• Hamada Nobushiro

  Division of Microbiology, Department of Infection Control, Kanagawa Dental College

• Shibata Takeshi

  Division of Fixed Prosthodontics, Department of Oral and Maxillofacial Rehabilitation, Kanagawa Dental College

• Hori Norio

  Division of Fixed Prosthodontics, Department of Oral and Maxillofacial Rehabilitation, Kanagawa Dental College

• Aoki Hiromichi

  Division of Fixed Prosthodontics, Department of Oral and Maxillofacial Rehabilitation, Kanagawa Dental College

• Nonami Toru

  Division of Removal Prosthetics, Department of Oral and Maxillofacial Rehabilitation, Kanagawa Dental College School of Life System Science and Technology Chukyo University

• Toyoda Minoru

  Division of Removal Prosthetics, Department of Oral and Maxillofacial Rehabilitation, Kanagawa Dental College

Abstract

Purpose: This study investigated the inhibitory effect of acrylic resin incorporating FAp-TiO₂ against the adhesion of C. albicans.<br>Materials and methods: FAp-TiO₂ was added at 1, 5, 10 wt% to acrylic resin to form specimen disks of 7 mm diameter, and the surface roughness was measured. Then, C. albicans was cultured aerobically at 37 °C for 24 hours in glucose peptone yeast extract (GPY) broth, and adjusted to OD=1 at 550 nm with a KCl-buffer. The disks were immersed in the C. albicans suspension for 2 hours at 37 °C with UVA from a black light source. After the incubation, the disks were washed gently with phosphate buffer saline (PBS), and the luminescent signal derived from the viable cells on the disks was determined using a bioluminescence adenosine triphosphate (ATP) assay.<br>Results: In the measurement of surface roughness, no significant difference was observed between any of the specimens. The adhesion assay of C. albicans revealed that a statistically significant decrease was observed in FAp-TiO₂ when compared with the control (p‹0.01).<br>Conclusion: We suggest that acrylic resin incorporating FAp-TiO₂ prevents the adhesion of C. albicans, indicating a wide range of possibilities for the practical use of new photocatalysts in acrylic resin.

Journal

• Prosthodontic Research & Practice

  Prosthodontic Research & Practice 7 (2), 201-203, 2008

  Japan Prosthodontic Society

Keywords

• TiO₂ photocatalyst
• fluoridated-apatite
• Candida albicans
• acrylic resin

Details

• CRID: 1390001205195650432
• NII Article ID: 10021955243
• NII Book ID: AA11728086
• DOI: 10.2186/prp.7.201
• ISSN: 13477021
• Web Site: http://www.jstage.jst.go.jp/article/prp/7/2/7_2_201/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009105281
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed