Effect of a Tablet Containing Probiotic Bacteria and Stomachic Herbs on Human Fecal Microbiota

  • Yonejima Yasunori
    Research and Development Department, Nitto Pharmaceutical Industries, Ltd. Laboratory of Animal Science, Kyoto Prefectural University
  • Mori Yoshiro
    Research and Development Department, Nitto Pharmaceutical Industries, Ltd.
  • Ushida Kazunari
    Laboratory of Animal Science, Kyoto Prefectural University

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Abstract

We evaluated the effects of a tablet containing Bacillus natto and Lactobacillus acidophilus as probiotics supplemented with three stomachic herbs on human fecal microbiota. Six healthy subjects ingested 3 tablets, 3 times a day after meals for 10 days. Fecal samples were collected before and after the administration period, and 1 week post administration. As shown by the TGGE images, the density of the bands identified with Bifidobacterium increased in four cases. This increase was confirmed by real-time PCR. It was observed that the densities of the bands identified with Haemophilus decreased, Ruminococcus decreased, Clostridium colinum decreased, Acidaminococcus increased and Megamonas increased individually.<br>

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