Immunoblot Analysis Using Recombinant Protein of the 180kD Bullous Pemphigoid Antigen NC16a Domain as an Aid to the Diagnosis of Atypical Subepidermal Autoimmune Bullous Skin Diseases

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著者

    • 橋本 隆 HASHIMOTO Takashi
    • Department of Dermatology, School of Medicine, Keio University Department of Dermatology, School of Medicine, Keio University
    • 松村 都江 MATSUMURA Kunie
    • Department of Dermatology, School of Medicine, Keio University Department of Dermatology, School of Medicine, Keio University
    • 河原 由恵 KAWAHARA Yoshie
    • Department of Dermatology, School of Medicine, Keio University Department of Dermatology, School of Medicine, Keio University
    • 大畑 恵之 OHATA Yoshiyuki
    • Department of Dermatology, School of Medicine, Keio University Department of Dermatology, School of Medicine, Keio University

抄録

We have recently developed a novel immunoblotting system using bacterial fusion protein containing the NC16a domain, the most immunogenic region, of the 180 kD bullous pemphigoid (BP) antigen, which was shown to be highly sensitive and specific method to detect the antigen and to be very useful for the diagnosis of BP. In this study, using this immunoblot assay, we investigated sera obtained from 26 patients who showed atypical clinical features. The sera of these cases showed in general very weak reactivity with the basement membrane zone on immunofluorescence, and very weak and variable results in detection of either the 230 kD or 180 kD BP antigen with immunoblotting of human epidermal extracts. However, with immunoblotting of the BP180 NC16a domain fusion protein, this protein was detected by 21 out of the 26 cases. These results indicated that this new assay system is the most sensitive method to detect BP antigen, and should be very useful for the diagnosis for these atypical cases.

収録刊行物

  • Keio journal of medicine  

    Keio journal of medicine 44(2), 62-66, 1995-06-01 

    The Keio Journal of Medicine

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各種コード

  • NII論文ID(NAID)
    10021984954
  • NII書誌ID(NCID)
    AA00710216
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00229717
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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