Rapid Reduction in Ryanodine Binding of Hippocampus CA1 in Cerebral Ischemia

この論文にアクセスする

この論文をさがす

著者

    • 近藤 太郎 KONDO Taro
    • Department of Neurology, School of Medicine, Keio University Department of Neurology, School of Medicine, Keio University
    • 小山 聡 KOYAMA Satoshi
    • Department of Neurology, School of Medicine, Keio University Department of Neurology, School of Medicine, Keio University
    • 傳法 倫久 DEMBO Tomohisa
    • Department of Neurology, School of Medicine, Keio University Department of Neurology, School of Medicine, Keio University
    • 福内 靖男 FUKUUCHI Yasuo
    • Department of Neurology, School of Medicine, Keio University Department of Neurology, School of Medicine, Keio University

抄録

Ryanodine receptors located on the sarcoplasmic or endoplasmic reticulum, play an important role in the regulation of the intracellular Ca<sup>2+</sup> level via the mechanism of Ca<sup>2+</sup>-induced Ca<sup>2+</sup> release (CICR). Perturbation of intracellular Ca<sup>2+</sup> regulation has been considered to be one of the most important mechanisms underlying acute ischemic neuronal damage. The ryanodine binding, an indicator of intracellular channels of CICR, and local cerebral blood flow (LCBF) were therefore examined at 15min post-ischemia in the gerbil brain. The autoradiographic method developed in our laboratory enabled us to determine both parameters within the same brain. Severe hemispheric cerebral ischemia was induced by occluding the right common carotid artery. LCBF was measured at the end of the experiment using [<sup>14</sup>C]iodoantipyrine method. The ryanodine binding was evaluated autoradiographically <i>in vitro</i> using [3H] ryanodine. A group of gerbils who underwent a sham procedure served as controls. LCBF was found to be significantly decreased in most cerebral regions on the occluded side. In contrast, a significant reduction in ryanodine binding was noted, only in the hippocampus CAI on the occluded side. Taken together, these findings indicate that the CICR in the hippocampus CAI may be especially susceptible to acute ischemic stress, and be closely associated with the pathophysiological mechanisms of the selective vulnerability of this region.

収録刊行物

  • Keio journal of medicine  

    Keio journal of medicine 46(2), 85-89, 1997-06-01 

    The Keio Journal of Medicine

参考文献:  20件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10021987884
  • NII書誌ID(NCID)
    AA00710216
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00229717
  • データ提供元
    CJP書誌  J-STAGE 
ページトップへ