Constitutive activity of inwardly rectifying K[+] channel at physiological 〔Ca〕i is mediated by Ca[2+]/CaMK 2 pathway in opossum kidney proximal tubule cells
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- Mori Yoshiaki
- Department of Physiology, Osaka Medical College
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- Yoshida Hideyo
- Department of Physiology, Osaka Medical College
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- Miyamoto Manabu
- Department of Physiology, Osaka Medical College
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- Sohma Yoshiro
- Department of Physiology, Osaka Medical College
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- Kubota Takahiro
- Department of Physiology, Osaka Medical College
書誌事項
- タイトル別名
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- Constitutive Activity of Inwardly Rectifying K+ Channel at Physiological [Ca]i Is Mediated by Ca2+/CaMK II Pathway in Opossum Kidney Proximal Tubule Cells
- Constitutive activity of inwardly rectifying K channel at physiological Ca i is mediated by Ca 2 CaMK 2 pathway in opossum kidney proximal tubule cells
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抄録
Using patch-clamp technique, we studied the role of the Ca2+/calmodulin kinase II (CaMK II)-mediated phosphorylation process on the K+ channel with an inward conductance of 90 pS in opossum kidney proximal tubule cells (OKPCs). The intracellular Ca2+ concentration ([Ca]i) was measured by use of the fluorescent dye fura 2. The following results were obtained: (i) In cell-attached patches, the channel activity was inhibited by a decrease in [Ca]i induced by perfusion with low Ca2+ (10−8 M), La3+ (100 μM), or EGTA/AM (100 μM) contained in the bath solution. The application of KN-62 (10 μM) or KN-93 (5 μM), inhibitors of CaMK II, also inhibited the channel activity. (ii) The membrane potential measured with nystatin-perforated patches was significantly decreased by the fall in [Ca]i induced by the perfusion with EGTA- or La3+-containing solution. Also, the application of KN-62 (10 μM) or KN-93 (5 μM) to the bath significantly decreased the membrane potential. (iii) In inside-out patches, the channel activity was significantly stimulated by the application of CaMK II (300 pM) at 10−7 M Ca2+ in the bath. Furthermore, the application of KN-62 (10 μM) to the bath significantly decreased the channel activity. Our findings show that the constitutive activity of inwardly rectifying K+ channel at physiological [Ca]i is mediated by the Ca2+/CaMK II pathway in OKPCs.<br>
収録刊行物
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- The Journal of Physiological Sciences
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The Journal of Physiological Sciences 58 (3), 199-207, 2008
一般社団法人 日本生理学会
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詳細情報 詳細情報について
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- CRID
- 1390282680228449408
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- NII論文ID
- 10022598267
- 130004466925
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- NII書誌ID
- AA12129145
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- ISSN
- 18806562
- 18806546
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- NDL書誌ID
- 9549386
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可