Significance of Molecular Diagnosis using Histopathological Specimens in Cestode Zoonoses

DOI Web Site 被引用文献12件 参考文献168件
  • Yamasaki Hiroshi
    Department of Parasitology, Asahikawa Medical College Department of Parasitology, National Institute of Infectious Diseases
  • Nakaya Kazuhiro
    Animal Laboratory for Medical Research, Asahikawa Medical College
  • Nakao Minoru
    Department of Parasitology, Asahikawa Medical College
  • Sako Yasuhito
    Department of Parasitology, Asahikawa Medical College
  • Ito Akira
    Department of Parasitology, Asahikawa Medical College

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Cestode zoonosis cases confirmed by PCR-based mitochondrial DNA analysis were investigated. The cestodiosis included taeniasis, cysticercosis, alveolar echinococcosis, cystic echinococcosis, sparganosis mansoni, diphyllobothriasis and diplogonoporiasis. DNA samples were extracted from the ethanol-fixed, formalin-fixed, paraffin-embedded sections, HE-stained, and the PAS- or acetocarmine-stained samples submitted for histopathology. For PCR-based analysis, cytochrome c oxidase subunit 1 and⁄or cytochrome b genes were amplified by multiplex PCR or conventional PCR coupled with DNA sequencing. Although DNA molecules were degraded in most formalin-fixed samples, smaller gene fragments were successfully amplified and the species causing cestodiosis could be identified by DNA sequence analysis of the amplicons. This review describes cestode zoonosis cases in which mitochondrial DNA analysis was useful not only for routine and retrospective diagnosis, but also for genetic polymorphism analysis and molecular identification of the species associated with pathogenicity. The significance of molecular diagnosis using histopathological specimens for cestode zoonoses is also discussed.

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