植物プランクトン種組成測定のための生体励起蛍光スペクトルの特性抽出

• 堀内 智啓

  アレックス電子株式会社

• 和田 章嗣

  東海大学海洋学部

• 千賀 康弘

  東海大学海洋学部

• 秋葉 龍郎

  独立行政法人 産業技術総合研究所 セルエンジニアリング研究部門

書誌事項

タイトル別名

• In vivo Fluorescence Excitation Spectra Characteristics of Phytoplankton for an Application of the Taxonomic Identification.
• ショクブツ プランクトン シュ ソセイ ソクテイ ノ タメノ セイタイ レイキ ケイコウ スペクトル ノ トクセイ チュウシュツ

抄録

In order to identify phytoplankton taxonomic compositions by optical characteristics, we examined a relationship between the pigment composition and the excitation fluorescence spectrum using purely cultured species (Cyanophyceae. Bacillariophyceae. Raphydophyceae. Chlorophyceae, Dinophyceae). Excitation spectra and phytoplankton pigments were measured by a spectrofluorometer and a RP-HPLC with a phtodiode array detector. Cyanophyceae showed a characteristic spectrum caused by phycobili-protein pigments (PB). The fluorescent intensity was extremely high from 545nm to 600 nm. and it varied depending on the ratio of phycocyanin and phycoerythrin. Bacillariophyceae. Raphydophyceae and Dinophyceae had a broad peak at around 530 nm. which reflected the existence of light-harvesting-carotenoids (LHCs) in chloroplast. On the other hand. Chlorophyceae did not have any peak at the longer wavelength region than 500 nm. because there is no PB and LHCs which absorbs light at the wavelength region. From these excitation fluorescent features, these five classes could be characterized to three groups; Cyanophyceae. Chlorophyceae. and carotenoid-contained class (e.g. Bacillariophyta, Raphydophyceae and Dinophyta). Finally, we resulted that three fluorescent intensity ratios: [f(490)/f (530)1. I f (545)/f (440)1 and [f (545)/f (440) 1. enabled the identification of the three groups. Our results showed that the fluorescence-excitation-spectra measurement was helpful to classify phytoplankton-taxa based on the pigments composition. This information will be appreciable to develop an in-situ excitation fluorometer for automatic identification of phytoplankton groups.

収録刊行物

• 海洋理工学会誌

  海洋理工学会誌 14 (1), 15-26, 2008

  海洋理工学会

キーワード

• Phytoplankton taxonomic composition
• Fluorescence excitation spectrum
• Pigment composition

詳細情報

• CRID: 1390001288123506688
• NII論文ID: 10024279114
• NII書誌ID: AA11607434
• DOI: 10.14928/amstec.14.1_15
• ISSN: 21883262
• ISSN: 13412752
• NDL書誌ID: 9621946
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9621946
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • CiNii Articles
  • Crossref
• 抄録ライセンスフラグ: 使用不可