Production Efficiency and Telomere Length of the Cloned Pigs Following Serial Somatic Cell Nuclear Transfer

• KUROME Mayuko

  Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University Institute of Molecular Animal Breeding and Biotechnology, Ludwig-Maximilians-University Munich

• HISATOMI Hisashi

  Laboratory of Cellular and Molecular Biochemistry, Department of Materials and Life Science, Seikei Universty

• MATSUMOTO Shirou

  Department of Pediatrics, Graduate School of Medical Science, Kumamoto University

• TOMII Ryo

  Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University

• UENO Satoshi

  Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University

• HIRUMA Katsumi

  Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University

• SAITO Hitoshi

  Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University

• NAKAMURA Kimitoshi

  Department of Pediatrics, Graduate School of Medical Science, Kumamoto University

• OKUMURA Kenji

  Department of Pediatrics, Graduate School of Medical Science, Kumamoto University

• MATSUMOTO Mitsuhito

  National Agricultural Research Center for Kyushu Okinawa Region

• KAJI Yuji

  National Agricultural Research Center for Kyushu Okinawa Region

• ENDO Fumio

  Department of Pediatrics, Graduate School of Medical Science, Kumamoto University

• NAGASHIMA Hiroshi

  Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University

抄録

The aim of the present study was to examine the production efficiency of cloned pigs by serial somatic cell nuclear transfer (SCNT) and to ascertain any changes in the telomere lengths of multiple generations of pigs. Using fetal fibroblasts as the starting nuclear donor cells, porcine salivary gland progenitor cells were collected from the resultant first-generation cloned pigs to successively produce second- and third-generation clones, with no significant differences in production efficiency, which ranged from 1.4% (2/140) to 3.3% (13/391) among the 3 generations. The average telomere lengths (terminal restriction fragment values) for the first, second and third generation clones were 16.3, 18.1 and 20.5 kb, respectively, and were comparable to those in age-matched controls. These findings suggest that third-generation cloned pigs can be produced by serial somatic cell cloning without compromising production efficiency and that the telomere lengths of cloned pigs from the first to third generations are normal.<br>

収録刊行物

• Journal of Reproduction and Development

  Journal of Reproduction and Development 54 (4), 254-258, 2008

  公益社団法人 日本繁殖生物学会

キーワード

• Aging
• Pig cloning
• Progenitor cell
• Somatic cell nuclear transfer (SCNT)
• Telomere

詳細情報

• CRID: 1390282681313514752
• NII論文ID: 10024289316
• NII書誌ID: AA10936678
• DOI: 10.1262/jrd.20038
• ISSN: 13484400
• ISSN: 09168818
• NDL書誌ID: 9621639
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9621639
• Web Site: http://www.jstage.jst.go.jp/article/jrd/54/4/54_20038/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
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