Desipramine Activated Bcl-2 Expression and Inhibited Lipopolysaccharide-Induced Apoptosis in Hippocampus-Derived Adult Neural Stem Cells

DOI Web Site 参考文献87件
  • Huang Yu-Yin
    Department of Anesthesiology, Taipei Veterans General Hospital and National Yang-Ming University, Taiwan Department of Anesthesiology, Cheng-Hsin Rehabilitation Medical Center, Taiwan Institute of Clinical Medicine, National Yang-Ming University, Taiwan
  • Peng Chi-Hsien
    Department of Ophthalmology, Taipei Veterans General Hospital and National Yang-Ming University, Taiwan Institute of Clinical Medicine, National Yang-Ming University, Taiwan Shin Kong Wu Ho-Su Memorial Hospital, Taiwan
  • Yang Yi-Ping
    Department of Pharmacy, Taipei Veterans General Hospital and National Yang-Ming University, Taiwan
  • Wu Chih-Chiau
    Department of Ophthalmology, Taipei Veterans General Hospital and National Yang-Ming University, Taiwan
  • Hsu Wen-Ming
    Department of Ophthalmology, Taipei Veterans General Hospital and National Yang-Ming University, Taiwan
  • Wang Hsiao-Jung
    Department of Pharmacy, Taipei Veterans General Hospital and National Yang-Ming University, Taiwan
  • Chan Kwok-Han
    Department of Anesthesiology, Taipei Veterans General Hospital and National Yang-Ming University, Taiwan
  • Chou Yi-Pen
    Department of Anesthesiology, Cheng-Hsin Rehabilitation Medical Center, Taiwan
  • Chen Shih-Jen
    Department of Ophthalmology, Taipei Veterans General Hospital and National Yang-Ming University, Taiwan
  • Chang Yuh-Lih
    Department of Pharmacy, Taipei Veterans General Hospital and National Yang-Ming University, Taiwan Department of Pharmacy, Chia Nan University of Pharmacy and Science, Taiwan

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Desipramine (DP) is a tricyclic antidepressant used for treating depression and numerous other psychiatric disorders. Recent studies have shown that DP can promote neurogenesis and improve the survival rate of hippocampal neurons. However, whether DP induces neuroprotection or promotes the differentiation of neural stem cells (NSCs) needs to be elucidated. In this study, we cultured NSCs derived from the hippocampal tissues of adult rats as an in vitro model to evaluate the modulation effect of DP on NSCs. First, we demonstrated that the expression of Bcl-2 mRNA and nestin in 2 μM DP-treated NSCs were up-regulated and detected by real-time reverse transcriptase polymerase chain reaction (RT-PCR). The results of Western blotting and immunofluorescent study confirmed that Bcl-2 protein expression was significantly increased in Day 3 DP-treated NSCs. Using the Bcl-2 small interfering RNA (siRNA) method, our results further showed that DP protects the lipopolysaccharide (LPS)-induced apoptosis in NSCs, in part by activating the expression of Bcl-2. Furthermore, DP treatment significantly inhibited the induction of proinflammatory factor interleukin (IL)-1β, IL-6, and tumor necrosis factor-α in the culture medium of LPS-treated NSCs mediated by Bcl-2 modulation. The results of high performance liquid chromatography coupled to electrochemical detection further confirmed that DP significantly increased the functional production of serotonin (26 ± 3.5 μM, DP-treated 96 h) and noradrenaline (50 ± 8.9 μM, DP-treated 96 h) in NSCs through activation of the MAPK/ERK pathway and partially mediated by Bcl-2. In conclusion, the present results indicate that DP can increase neuroprotection ability by inhibiting the LPS-induced inflammatory process in NSCs via the modulation of Bcl-2 expression, as confirmed by the siRNA method.<br>

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