書誌事項
- タイトル別名
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- <i>In Vivo</i> Electroporation: A Convenient Method for Gene Transfer to Testicular Cells in Mice
- In Vivo Electroporation A Convenient Me
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抄録
In the present study, transfection efficiency of the chloramphenicol acetyltransferase reporter gene was compared in mouse testicular cells by utilizing lipofection, electroporation (EP) and microparticle bombardment (MPB) techniques in both in vivo and in vitro environments. In addition, in vivo EP was conducted to attain spatial expression of the bacterial β-galactosidase reporter gene in the testis of living mice. Altogether three experiments were conducted with male ICR strain mice at 4 weeks of age. The results showed that among the three different transfection methods employed, in vivo EP and in vivo MPB were the most efficient. When the in vivo EP technique was used to transfect the bacterial β-galactosidase reporter gene, spatial gene expression was clearly demonstrated by X-gal staining in the mouse testis where among X-gal stained cells, some spermatocyte- or spermatid-like cells were also found. By taking account of maximum amounts of transfectable DNA, the extent of tissue damage, and transfection efficiency, it was concluded that in vivo EP would provide a powerful and useful means to transfer foreign genes to testicular cells of living mice.
収録刊行物
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- 日本畜産学会報
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日本畜産学会報 67 (11), 975-982, 1996
公益社団法人 日本畜産学会
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詳細情報 詳細情報について
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- CRID
- 1390282680173644928
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- NII論文ID
- 10024585129
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- NII書誌ID
- AN00195188
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- ISSN
- 18808255
- 00215309
- 1346907X
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- NDL書誌ID
- 4088266
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可