Improvement of Biocompatibility of Chitosan Fiber Modified by Ca-Phosphate Deposition through an Alternate Soaking Process

• Yoshioka Tomohiko

  Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology

• Onomoto Hiroshi

  Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology

• Kashiwazaki Haruhiko

  Department of Gerodontology, Division of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University

• Inoue Nobuo

  Department of Gerodontology, Division of Oral Health Science, Graduate School of Dental Medicine, Hokkaido University

• Koyama Yoshihisa

  Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University

• Takakuda Kazuo

  Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University

• Tanaka Junzo

  Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology

抄録

A tube consisted of chitin/chitosan nanofibers is one of the candidates to support nerve regeneration. However, chitin/chitosan would cause inflammation when implanted in human body. This study indicates that chitosan nanofibers (degree of deacetylation; 78% and 93%) could be modified by alternate soaking in calcium and phosphate solutions, resulting in the improvement of their biocompatibility in terms of both in vitro cell culture experiment and in vivo animal test. Deacetylation of the chitosan nanofibers appeared to have little effect on in vitro and in vivo behaviors after 3 days. However, the alternate soaking process clearly improved in vitro and in vivo responses against the chitosan nanofibers. During the process, the surface morphology of the chitosan nanofiber was not changed, i.e. no hydroxyapatite was deposited due to the limited number of soaking. Thus, the surface chemical and physical states of the modified chitosan nanofiber such as surface charge, surface free-energy, and wettability was found to influence both the cell attachment and inflammation.

収録刊行物

• MATERIALS TRANSACTIONS

  MATERIALS TRANSACTIONS 50 (6), 1269-1272, 2009

  公益社団法人 日本金属学会

キーワード

• chitosan fiber
• electrospinning
• alternate soaking process
• vascular endothelial cell
• inflammation

詳細情報

• CRID: 1390001204250240640
• NII論文ID: 10024815520
• NII論文ID: 130004454274
• NII書誌ID: AA1151294X
• DOI: 10.2320/matertrans.me200826
• ISSN: 13475320
• ISSN: 13459678
• NDL書誌ID: 10236875
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10236875
• Web Site: https://www.jstage.jst.go.jp/article/matertrans/50/6/50_ME200826/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可