Effect of intraperitoneal needling on pancreatic β-cell cytotoxicity mediated via alloxan in mice with an FVB/N genetic background

• AMAO Hiromi

  Laboratory of Experimental Animal Science, Nippon Veterinary and Life Science University

• IWAMOTO Rena

  Laboratory of Experimental Animal Science, Nippon Veterinary and Life Science University

• KOMUKAI Yumi

  Laboratory of Experimental Animal Science, Nippon Veterinary and Life Science University

• DOBASHI Yuu

  Laboratory of Experimental Animal Science, Nippon Veterinary and Life Science University

• TAKAHASHI Kimimasa

  Laboratory of Veterinary Pathology, Nippon Veterinary and Life Science University

• TOHEI Atsushi

  Laboratory Animal Research Center, Dokkyo Medical University

• NAKAMA Kazumasa

  Laboratory of Experimental Animal Science, Nippon Veterinary and Life Science University

• TOYAMA-HONDA Kiyoko

  Unité de Neurobiologie de la Croissance et de la Sénescence, INSERM, Centre Paul Broca

書誌事項

タイトル別名

• Effect of Intraperitoneal Needling on Pancreatic .BETA.-Cell Cytotoxicity Mediated via Alloxan in Mice with an FVB/N Genetic Background
• Effect of intraperitoneal needling on pancreatic v cell cytotoxicity mediated via alloxan in mice with an FVB N genetic background

抄録

The present study investigated whether pre-stimulation with intraperitoneal (i.p.) needling protects against development of diabetes in alloxan-treated transgenic (Tg) mice overexpressing the human Cu/Zn superoxide dismutase gene or non-Tg littermates of the FVB/N strain. Twenty minutes before the alloxan treatment (60mg/kg) the mice were injected intraperitoneally with 0.05ml saline while control mice received only the alloxan treatment. Hyperglycemic responses of the saline-injected mice to alloxan were significantly suppressed in the Tg mice (P<0.05). A similar reduction of response was also observed in non-Tg littermates, but the effect was less than that in the Tg mice. This protective effect on the diabetogenic action of alloxan was also demonstrated by an analysis of the number of days positive for urinary glucose, and by immunohistochemical analysis of pancreatic insulin-positive cells. A similar suppressive effect on the hyperglycemic response of alloxan was observed in the mice stimulated by i.p. needling alone. However, suppression of the hyperglycemic response was not observed in ICR mice receiving an i.p. injection. These results suggest that the diabetogenic action of alloxan can be suppressed by i.p. needling-mediated stimulation in mice that have a genetic background of the FVB/N strain. Since a slight protective effects of alloxan-induced diabetes was also observed in the Tg mice compared to FVB/N mice treated with only alloxan, this phenomenon could be more clearly seen in the Tg mice than in non-Tg littermates with an FVB/N background.<br>

収録刊行物

• Experimental Animals

  Experimental Animals 58 (2), 151-158, 2009

  公益社団法人 日本実験動物学会

キーワード

• alloxan
• Cu/Zn superoxide dismutase
• FVB/N mice
• intraperitoneal needling
• pancreatic β-cell cytotoxicity

詳細情報

• CRID: 1390282680021709440
• NII論文ID: 130000122129
• NII論文ID: 10024838317
• NII書誌ID: AA11032321
• DOI: 10.1538/expanim.58.151
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL書誌ID: 10206575
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10206575
• Web Site: http://www.jstage.jst.go.jp/article/expanim/58/2/58_2_151/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009252208
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可