Determination of Appropriate Activation Time and Timing in Round Spermatid Injection into Mouse Oocytes, and Chromosomal Analysis of Resultant Embryos

• Tsurumaki Chiaki

  Graduate School of Agricultural, Utsunomiya University

• Mitsui Akinori

  United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology

• Matsumoto Hiromichi

  Graduate School of Agricultural, Utsunomiya University

• Fukui Emiko

  Graduate School of Agricultural, Utsunomiya University

• Yoshizawa Midori

  Graduate School of Agricultural, Utsunomiya University United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology

Bibliographic Information

Other Title

• マウス円形精子細胞卵子内注入法（Round spermatid injection；ROSI）における卵子活性化処理と胚の細胞遺伝学的正常性の検討
• マウス エンケイ セイシ サイボウ ランシ ナイ チュウニュウホウ Round spermatid injection ROSI ニ オケル ランシ カッセイカ ショリ ト ハイ ノ サイボウ イデンガクテキ セイジョウセイ ノ ケントウ

Abstract

The present study focused on the level of Sr²⁺ activation required by mouse oocytes in round spermatid injection (ROSI) and analyzed the resultant embryos cytogenetically at the first cleavage and blastocyst stages. Mouse oocytes were divided into 3 groups: Group A, oocytes treated by activation using Sr²⁺ for 40 min before ROSI; Group B, oocytes treated by activation using Sr²⁺ for 1 h after ROSI; and Group C, oocytes treated by activation using Sr²⁺ for 5 h after ROSI. One round spermatid obtained from mature RFM/Ms-Rb(6.15) males was injected into each oocyte individually. The fertilization rate of oocytes in ROSI, the development rate of zygotes to the blastocyst stage and chromosomal normality in the resultant embryos were highest in Group A, suggesting activation using Sr²⁺ for 40 min before ROSI as the most appropriate treatment. At the first cleavage, many kinds of male-derived abnormalities, such as asynchrony and constitutive chromosome abnormalities etc., were observed in all groups. At the blastocyst stage, many parthenogenetic embryos, showing n, 2n and 2n/n with no translocated chromosome, were typically observed in all groups. Normal offspring were obtained by embryo transfer of the blastocysts derived from Group A, and their fertility after sexual maturity was confirmed by mating.<br>

Journal

• Journal of Mammalian Ova Research

  Journal of Mammalian Ova Research 26 (2), 86-93, 2009

  JAPANESE SOCIETY OF OVA RESEARCH

Keywords

• Mouse
• ROSI
• Chromosome
• Oocytes activation
• Parthenogenesis

Details

• CRID: 1390282679316224512
• NII Article ID: 10024964817
• NII Book ID: AN10548943
• DOI: 10.1274/jmor.26.86
• ISSN: 13475878
• ISSN: 13417738
• NDL BIB ID: 10287394
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010772492
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10287394
• Text Lang: ja
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed