Selection of synonymous codons for better expression of recombinant proteins in tobacco chloroplasts

• Nakamura Masayuki

  Graduate School of Natural Sciences, Nagoya City University

• Sugiura Masahiro

  Graduate School of Natural Sciences, Nagoya City University Sugiyama Human Research Center, Sugiyama Jogakuen University

抄録

The 20 amino acids, except for methionine and tryptophan, are coded for by two to six codons called synonymous codons. Synonymous codons are used differently by different organisms. Hence, codon selection should be important to express recombinant proteins in tobacco chloroplasts. We present here the codon usage table from the entire 79 mRNAs from tobacco chloroplasts. As codons function on mRNAs, codon usage tables should be constructed from mRNAs but not from genes. We devised an in vitro assay for translation efficiencies of codons, and measured the translation efficiencies of several synonymous codon groups in tobacco chloroplasts. Our results indicated that translation efficiencies of individual codons are not always correlated with codon usage. Based on these data, we discuss on synonymous codon selection for expression of inserted genes in tobacco chloroplasts.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 26 (1), 53-56, 2009

  日本植物バイオテクノロジー学会

キーワード

• Chloroplast
• codon usage
• synonymous codon
• tobacco
• translation efficiency

詳細情報

• CRID: 1390282679305522560
• NII論文ID: 10025181202
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.26.53
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 10196871
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10196871
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可