Proteome analysis of silkworm 2. Hemolymph

• Kajiwara Hideyuki

  National Institute of Agrobiological Sciences

• Imamaki Atsue

  Environmental Research Center

• Nakamura Masatoshi

  National Institute of Agrobiological Sciences

• Mita Kazuei

  National Institute of Agrobiological Sciences

• Xia Qingyu

  Institute of Sericulture and Systems Biology, Southwest University

• Ishizaka Masami

  National Institute for Agro-Environmental Sciences

抄録

Proteomic analysis of silkworm hemolymph at the fifth-instar day-3 larva stage was performed by combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Prominent spots on 2D-gel were analyzed after trypsinization and proteins were identified by Mascot and Sequest using the amino acid sequence data obtained from the genome sequence of silkworm. The gel images were tracked from the fourth-instar day-1 larva stage to the stage before adult moth for twenty-one days. The results of mass spectrometry analysis and changes in gel images are summarized in the silkworm proteome database (http://kaiko2ddb.dna.affrc.go.jp/cgi-bin/search_2DDB.cgi).<br>

収録刊行物

• Journal of Electrophoresis

  Journal of Electrophoresis 53 (2), 27-31, 2009

  日本電気泳動学会

キーワード

• hemolymph
• mass spectrometry
• proteome
• silkworm (<i>Bombyx mori</i> L.)
• two-dimensional polyacrylamide gel electrophoresis

詳細情報

• CRID: 1390282680204410624
• NII論文ID: 10025192018
• NII書誌ID: AA12015033
• DOI: 10.2198/jelectroph.53.27
• COI: 1:CAS:528:DC%2BD1MXnsl2lsr4%3D
• ISSN: 13499408
• ISSN: 13499394
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可