Analysis of Saccharides Possessing Post-translational Protein Modifications by Phenylhydrazine Labeling Using High-Performance Liquid Chromatography

• SUZUKI Hiroshi

  Hitachi High-Technologies Corporation

• KATO Eri

  Hitachi High-Tech Manufacturing & Service Corporation

• MATSUZAKI Ayako

  Hitachi High-Technologies Corporation

• ISHIKAWA Masako

  Hitachi High-Technologies Corporation

• HARADA Yushi

  Hitachi High-Technologies Corporation

• TANIKAWA Kenichi

  Hitachi High-Technologies Corporation

• NAKAGAWA Hiroaki

  Hitachi High-Technologies Corporation

抄録

Sugar analysis by postcolumn labeling using phenylhydrazine with high-performance liquid chromatography is herein introduced. Phenylhydrazine reacts not only with the reducing end of oligosaccharides, but also with mono-sugar components with degraded glycosyl bonds. N-Acethylhexosamine is highly sensitive at a sub-pico molecular range, and this method is useful for detecting glycans on glycoproteins. The detection limit of fetuin, a protein possessing 3 N-glycans and 3 O-glycans, was 0.17 pmol by infusion analysis, and chromatograms of glycopeptides separated on an ODS column were obtained. These results suggest that this method is useful for the preparation of glycopeptides for glyco-proteomics research.

収録刊行物

• Analytical Sciences

  Analytical Sciences 25 (8), 1039-1042, 2009

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390001204256727936
• NII論文ID: 10025214242
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.25.1039
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 10304585
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10304585
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.25.1039.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.25.1039/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/25/8/25_8_1039/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可