Evaluation of Identification Methods for Staphylococcus aureus and Development of Modified Detection Methods for Staphylococcal Enterotoxin A

• SHIMAMURA Yuko

  Department of Nutrition and Food Science, Ochanomizu University

• MURATA Masatsune

  Department of Nutrition and Food Science, Ochanomizu University

抄録

We evaluated whether the agar plate method using a Baird Parker + rabbit plasma fibrinogen (RPF) agar could be used in place of the combination of a latex agglutination test (PS-latex) and a tube coagulase test for identification of Staphylococcus aureus from other strains of staphylococci. The PS-latex and tube coagulase tests showed positive results for 96 (100%) and 87 (90.6%) S. aureus strains, respectively. However, the PS-latex test showed positive results for 32 non-S. aureus strains. On the other hand, the agar plate method was positive for all the strains of S. aureus and showed no false-positive or -negative results. Next, we developed a highly sensitive and selective enzyme-linked immunosorbent assay (ELISA) for the detection of staphylococcal enterotoxin A (SEA). The interference by protein A and an unknown protein were eliminated using proteolytic digestion. This improved method is useful for SEA detection.

収録刊行物

• Food Science and Technology Research

  Food Science and Technology Research 15 (1), 89-94, 2009

  公益社団法人 日本食品科学工学会

キーワード

• coagulase
• proteolytic digestion
• staphylococcal enterotoxin A
• <i>Staphylococcus aureus</i>

詳細情報

• CRID: 1390282679431502464
• NII論文ID: 10025348644
• NII書誌ID: AA11320122
• DOI: 10.3136/fstr.15.89
• ISSN: 18813984
• ISSN: 13446606
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可