MIN6 is not a pure β cell line but a mixed cell line with other pancreatic endocrine hormones.

• NAKASHIMA Koji

  Diabetes and Endocrine Division, Department of Medicine, Kawasaki Medical School

• KANDA Yukiko

  Diabetes and Endocrine Division, Department of Medicine, Kawasaki Medical School

• HIROKAWA Yasushi

  Diabetes and Endocrine Division, Department of Medicine, Kawasaki Medical School

• KAWASAKI Fumiko

  Diabetes and Endocrine Division, Department of Medicine, Kawasaki Medical School

• MATSUKI Michihiro

  Diabetes and Endocrine Division, Department of Medicine, Kawasaki Medical School

• KAKU Kohei

  Diabetes and Endocrine Division, Department of Medicine, Kawasaki Medical School

書誌事項

タイトル別名

• MIN6 Is Not a Pure Beta Cell Line but a Mixed Cell Line with Other Pancreatic Endocrine Hormones

抄録

MIN6 cells retains glucose-stimulated insulin secretion (GSIS) as isolated islets. We comprehensively evaluated the gene expression and production of other islet hormones in MIN6 cells. Islet hormones were demonstrated by immunohistochemical staining and measured by ELISA. The gene expression profiles of MIN6 cells were compared with those in the mouse islets obtained by the laser capture micro-dissection (LCM). MIN6 cells excreted insulin, glucagon, somatostatin and ghrelin. They expressed mRNAs of insulin I and II, proglucagon, somatostatin, pancreatic polypeptide (PP) and ghrelin which were shown in the mouse pancreatic islet core and periphery obtained by LCM. A variety of genes closely related to the islet hormone producing cells were expressed in MIN6. Confocal laser scanning microscopy revealed that MIN6 cells included not only insulin positive cells but also insulin and glucagon or somatostin double positive cells. Glucagon, somatostatin and ghrelin were detectable in the culture medium. The present study clearly demonstrated that MIN6 produce pancreatic endocrine cells. It would be possible to use this cell line as a model to research the development, cell differentiation and function of pancreatic islets.<br>

収録刊行物

• Endocrine Journal

  Endocrine Journal 56 (1), 45-53, 2009

  一般社団法人 日本内分泌学会

キーワード

• MIN6
• Islet
• Insulin
• Glucagon
• Somatostatin

詳細情報

• CRID: 1390282681277562880
• NII論文ID: 10025610156
• NII書誌ID: AA10901436
• DOI: 10.1507/endocrj.k08e-172
• ISSN: 13484540
• ISSN: 09188959
• Web Site: http://www.jstage.jst.go.jp/article/endocrj/56/1/56_K08E-172/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2009231026
• 本文言語コード: en
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