Ca〔2+〕-Dependent Induction of Intracellular Ca〔2+〕 Oscillation in Hippocampal Astrocytes During Metabotropic Glutamate Receptor Activation

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  • Yoshida Yoshitoku
    Molecular Life Science Division, School of Life Science, Tokyo University of Pharmacy and Life Science
  • Tsuchiya Remi
    Molecular Life Science Division, School of Life Science, Tokyo University of Pharmacy and Life Science
  • Matsumoto Nagisa
    Molecular Life Science Division, School of Life Science, Tokyo University of Pharmacy and Life Science
  • Morita Mitsuhiro
    Molecular Life Science Division, School of Life Science, Tokyo University of Pharmacy and Life Science
  • Miyakawa Hiroyoshi
    Molecular Life Science Division, School of Life Science, Tokyo University of Pharmacy and Life Science
  • Kudo Yoshihisa
    Molecular Life Science Division, School of Life Science, Tokyo University of Pharmacy and Life Science

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タイトル別名
  • Ca2+-Dependent Induction of Intracellular Ca2+ Oscillation in Hippocampal Astrocytes During Metabotropic Glutamate Receptor Activation
  • Ca<sup>2+</sup>-dependent induction of intracellular Ca<sup>2+</sup> oscillation in hippocampal astrocytes during metabotropic glutamate receptor activation

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We have investigated whether the intracellular calcium concentration ([Ca2+]i) oscillations induced in astrocytes using the metabotropic glutamate-receptor agonist, (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (t-ACPD) are Ca2+-dependent, using three different Ca2+ indicators with different affinities for Ca2+. When rat hippocampal cells in culture were loaded with fura-2 (Kd: 145 nM), two-thirds of the cells showed obvious oscillatory increase in [Ca 2+]i during t-ACPD-administration. Those cells were identified as astrocytes by immuno-histochemistry in our previous paper. In cells loaded with fura-2FF (Kd: 25,000 nM), a similar percentage of t-ACPD-responsive cells showed oscillatory [Ca2+]i changes. However, in cells loaded with quin-2 (Kd: 60 nM), t-ACPD induced no oscillatory responses, but some cells showed a small transient increase in the [Ca2+]i. The same small transient [Ca2+]i increase was seen in cells loaded with both fura-2FF and BAPTA, a Ca2+ chelator (Kd: 135 nM). These findings indicate the involvement of [Ca2+]i-dependent regulatory mechanisms in the induction of the t-ACPD-induced oscillatory change in the [Ca2+]i in astrocytes.<br>

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