Simple and Noninvasive Breath Test Using 13C-Acetic Acid to Evaluate Gastric Emptying in Conscious Rats and Its Validation by Metoclopramide

• Uchida Masayuki

  Food Science Institute, Division of Research and Development, Meiji Dairies Corporation

• Endo Noriko

  Food Science Institute, Division of Research and Development, Meiji Dairies Corporation

• Shimizu Kimiko

  Food Science Institute, Division of Research and Development, Meiji Dairies Corporation

Abstract

The ¹³C-breath test has been used to clinically evaluate gastric emptying. However, this method has not been sufficiently validated in experimental animals. The present study aimed to establish a simple and noninvasive ¹³C-breath-test system in Sprague-Dawley male rats. After fasting, rats were orally administered Racol containing ¹³C-acetic acid and housed in a desiccator. The expired air in the chamber was collected in a breath-sampling bag using a tube and aspiration pump. The level of ¹³CO₂ in the expired air was measured using an infrared spectrometer at appropriate intervals for 120 min. During this period, the rate of ¹³CO₂ excretion increased, peaked, and decreased thereafter. The maximum concentration (C_max) and area under the curve (AUC_{120 min}) of ¹³CO₂ excretion increased in volume- and dose-dependent manners. The time taken to reach the maximum concentration (T_max) of ¹³CO₂ excretion increased as the volume increased, but was not affected by the dose of ¹³C-labeled acetic acid. Metoclopramide dose-dependently increased the C_max and shortened T_max of ¹³CO₂ excretion compared with those of the control rats, whereas the AUC_{120 min} was not affected. These results confirm that this simple method can successfully evaluate gastric emptying. Moreover, this system is suitable for investigating additional physiological functions using other labeled compounds.<br>

Journal

• Journal of Pharmacological Sciences

  Journal of Pharmacological Sciences 98 (4), 388-395, 2005

  The Japanese Pharmacological Society

Keywords

• ¹³C-acetic acid
• breath test in rats
• gastric emptying
• metoclopramide

Details

• CRID: 1390282680153196800
• NII Article ID: 10025729545
• NII Book ID: AA11806667
• DOI: 10.1254/jphs.fp0050153
• COI: 1:CAS:528:DC%2BD2MXps1OmtLY%3D
• ISSN: 13478648
• ISSN: 13478613
• ISSN: http://id.crossref.org/issn/0015749X
• NDL BIB ID: 7402275
• PubMed: 16082175
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7402275
• Web Site: https://search.jamas.or.jp/link/ui/2006234014
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed