Comparative Study of Calcium Ion Dynamics and Contractile Response in Rat Middle Cerebral and Basilar Arteries

• Hashimoto Terumasa

  Department of Pharmacology, School of Pharmaceutical Sciences, Showa University, Japan

• Ohata Hisayuki

  Department of Pharmacology, School of Pharmaceutical Sciences, Showa University, Japan

• Nobe Koji

  Department of Pharmacology, School of Pharmaceutical Sciences, Showa University, Japan

• Honda Kazuo

  Department of Pharmacology, School of Pharmaceutical Sciences, Showa University, Japan

抄録

The objective of this study was to compare intracellular calcium concentration ([Ca²⁺]_i) and contractile responses in isolated rat middle cerebral artery (MCA) with those in basilar artery (BA) employing real-time confocal laser microscopy. KCl elicited transient [Ca²⁺]_i elevation and sustained contraction in both arteries; moreover, nearly equal responses were evident in both arteries. Application of 5-hydroxytryptamine (5-HT), vasopressin (VP), and α,β-methylene adenosine 5'-triphosphate (α,β-me ATP) also induced elevation of [Ca²⁺]_i and contraction in both arteries. The maximum response of 5-HT and VP necessary to increase [Ca²⁺]_i and to constrict the MCA was less in comparison to the BA; however, a linear relationship emerged between the maximum response of [Ca²⁺]_i and that of contraction. Additionally, the slope of the correlation regression line of MCA was nearly identical to that of BA. On the other hand, cyclopiazonic acid (CPA)-induced Ca²⁺ release from store sites following contraction of MCA was distinct from that of BA. In MCA, velocity of [Ca²⁺]_i elevation in smooth muscle cells and Ca²⁺-wave propagation along smooth muscle cells induced by 5-HT were slower than those in BA. These observations revealed that different regions of arteries along the same cerebral tissue may display distinct [Ca²⁺]_i response; moreover, this difference may be one reason for the distinct contractile response.<br>

収録刊行物

• Journal of Pharmacological Sciences

  Journal of Pharmacological Sciences 109 (4), 546-555, 2009

  公益社団法人 日本薬理学会

キーワード

• confocal microscopy
• intracellular calcium
• basilar artery
• middle cerebral artery

詳細情報

• CRID: 1390001205180638464
• NII論文ID: 10025735826
• NII書誌ID: AA11806667
• DOI: 10.1254/jphs.08310fp
• ISSN: 13478648
• ISSN: 13478613
• NDL書誌ID: 10209729
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10209729
• Web Site: https://search.jamas.or.jp/link/ui/2009275457
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可