A Modified Warthin-Starry Silver Impregnation Method to Detect Programmed Cell Death in the Interdigital Tissue of the Fetal Rat Limb

• Takeda Tsuyoshi

  Toxicological Research Laboratories, KYOWA HAKKO KOGYO CO., LTD.

• Takaba Katsumi

  Toxicological Research Laboratories, KYOWA HAKKO KOGYO CO., LTD.

• Saeki Koji

  Toxicological Research Laboratories, KYOWA HAKKO KOGYO CO., LTD.

• Takahashi Manabu

  Toxicological Research Laboratories, KYOWA HAKKO KOGYO CO., LTD. Professor Emeritus, Yamaguchi University School of Medicine

• Kimoto Naoya

  Toxicological Research Laboratories, KYOWA HAKKO KOGYO CO., LTD.

• Kakuni Masakazu

  Toxicological Research Laboratories, KYOWA HAKKO KOGYO CO., LTD.

• Ikegami Jiro

  Toxicological Research Laboratories, KYOWA HAKKO KOGYO CO., LTD.

• Suzuki Kazuo

  Toxicological Research Laboratories, KYOWA HAKKO KOGYO CO., LTD.

• Sato Hitoshi

  Toxicological Research Laboratories, KYOWA HAKKO KOGYO CO., LTD.

• Mizutani Masato

  Toxicological Research Laboratories, KYOWA HAKKO KOGYO CO., LTD.

• Kojima Seiji

  Toxicological Research Laboratories, KYOWA HAKKO KOGYO CO., LTD.

抄録

Previously we have shown that a modified Warthin-Starry (mWS) method, to which was added a minor change to prevent fading, is useful and convenient to detect chemically induced chromatin condensation during thymic apoptosis in rats treated with dexamethasone. In this study, we investigated the relationship between mWS-positive cells and programmed cell death in the interdigital spaces of the developing limb in fetal SD rats. The limbs were removed from fetuses at day 15 of gestation, and routinely processed for both hematoxylin and eosin (H&E) staining and the mWS method. The results clearly showed that the distribution pattern of mWS-positive cells coincided well with that for the programmed cell death observed by H&E staining in the mesenchyme of interdigital notches and at the areas corresponding to the interphalangeal joints. These results confirm that the mWS method might be useful and convenient to detect condensed chromatin during apoptosis in embryonic organogenesis as well as by chemical induction.<br>

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃ　Ｐａｔｈｏｌｏｇｙ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃ　Ｐａｔｈｏｌｏｇｙ 16 (3), 187-190, 2003

  日本毒性病理学会

キーワード

• silver staining
• programmed cell death
• fetal limb

詳細情報

• CRID: 1390282679391717248
• NII論文ID: 10025851008
• NII書誌ID: AA12022786
• DOI: 10.1293/tox.16.187
• ISSN: 1881915X
• ISSN: 09149198
• Web Site: http://www.jstage.jst.go.jp/article/tox/16/3/16_3_187/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2004123644
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可