Effect of Gentamicin on Growth and Differentiation of Human Mesenchymal Stem Cells
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- Kagiwada Harumi
- Tissue Engineering Research Group, Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST)
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- Fukuchi Takeshi
- Life Science Laboratories, Life Science RD Center, KANEKA Corporation
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- Machida Hiroko
- Tissue Engineering Research Group, Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST)
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- Yamashita Kenji
- Life Science Laboratories, Life Science RD Center, KANEKA Corporation
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- Ohgushi Hajime
- Tissue Engineering Research Group, Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST)
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抄録
A tissue engineering approach using human mesenchymal stem cells (MSCs) can be used to regenerate bone/cartilage tissue. For effective tissue regeneration, cell cultivation under biologically safe conditions is essential. The objective of this study was to determine suitable antibiotic concentrations in culture media for MSCs while maintaining their inherent capabilities for proliferation and osteogenic/chondrogenic differentiation. When human MSCs were cultured with over 200 μg/mL of gentamicin, the rate of cell growth decreased significantly. In contrast, a concentration of 20 μg/mL gentamicin supported cell proliferation and differentiation capability very well and did not change the expression patterns of cell surface antigens (CD44+, CD105+, CD34-, CD45-). The concentration of 20 μg/ml is much higher than that of the MIC (Minimum Inhibition Concentration) of common bacteria, such as Staphylococcus aureus, Haemophilus influenzae and Escherichia coli. Therefore, we recommend 20 μg/mL of gentamicin in culture media for effective tissue regeneration to avoid bacterial contamination and maintain the osteogenic/chondrogenic capability of MSCs. <br>
収録刊行物
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- Journal of Toxicologic Pathology
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Journal of Toxicologic Pathology 21 (1), 61-67, 2008
日本毒性病理学会
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詳細情報 詳細情報について
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- CRID
- 1390282679392281856
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- NII論文ID
- 10025855658
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- NII書誌ID
- AN10232280
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- ISSN
- 1881915X
- 09149198
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- NDL書誌ID
- 9463374
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可