Immunohistochemicalanalysisofosteoconductivityof β-tricalciumphosphate and carbonate apatite applied in femoral and parietal bone defects in rats

• TAKEUCHI Hiroshi

  Division of Oral Pathology, Department of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry

• NAGAYAMA Motohiko

  Division of Oral Pathology, Department of Oral Pathogenesis and Disease Control, Asahi University School of Dentistry

• IMAIZUMI Yoshinori

  Department of Orthopedic Surgery, Murakami Memorial Hospital, Asahi University

• TSUKAHARA Takashi

  Department of Orthopedic Surgery, Murakami Memorial Hospital, Asahi University

• NAKAZAWA Junko

  Department of Orthopedic Surgery, Murakami Memorial Hospital, Asahi University

• KUSAKA Yoshiaki

  Department of Orthopedic Surgery, Murakami Memorial Hospital, Asahi University

• OHTOMO Katsuyuki

  Department of Orthopedic Surgery, Murakami Memorial Hospital, Asahi University

書誌事項

タイトル別名

• Immunohistochemical analysis of osteoconductivity of .BETA.-tricalcium phosphate and carbonate apatite applied in femoral and parietal bone defects in rats

抄録

The feature of osteoconductivity, and expression of inductive BMP and transcription factors (Runx2 and Osterix) for osteoblast differentiation, which was related to conductive bone formation, were observed in experimentally created defects in rat femoral and parietal bones filled with β-tricalcium phosphate (β-TCP) or carbonate apatite (CAP). Femoral cortical bone defects were repaired by conductive bone formed by osteoblasts differentiated around β-TCP and CAP, and immunohistochemical observation revealed that the osteoblasts expressed BMPs, Runx2, and Osterix. However, the repair in parietal bone defects was incomplete despite the β-TCP and CAP filling. Only cells, which differentiated around β-TCP or CAP, and formed conductive bone expressed BMPs, Runx2, and Osterix. These findings revealed that the osteoconductivity of calcium phosphate materials required the expression of BMPs as the prerequisite for Runx2 and Osterix expression. Therefore, it is suggested that when calcium phosphate ceramics are used as bone substitute materials, BMPs are essential for osteoconductivity.

収録刊行物

• Dental Materials Journal

  Dental Materials Journal 28 (5), 595-601, 2009

  一般社団法人　日本歯科理工学会

キーワード

• Calcium phosphate
• Osteoconductivity
• Immunohistochemistry

詳細情報

• CRID: 1390001204715855744
• NII論文ID: 10025916703
• NII書誌ID: AA10443149
• DOI: 10.4012/dmj.28.595
• COI: 1:STN:280:DC%2BD1Mnos1Skug%3D%3D
• ISSN: 18811361
• ISSN: 02874547
• PubMed: 19822991
• Web Site: https://www.jstage.jst.go.jp/article/dmj/28/5/28_5_595/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010070732
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可