Distribution of N-Acylhomoserine Lactone-Producing Fluorescent Pseudomonads in the Phyllosphere and Rhizosphere of Potato (Solanum tuberosum L.)

• Someya Nobutaka

  National Agricultural Research Center for Hokkaido Region (NARCH), National Agriculture and Food Research Organization (NARO)

• Morohoshi Tomohiro

  Department of Applied Chemistry, Utsunomiya University

• Okano Nobuya

  Department of Applied Chemistry, Utsunomiya University

• Otsu Eiko

  National Agricultural Research Center for Hokkaido Region (NARCH), National Agriculture and Food Research Organization (NARO)

• Usuki Kazuei

  National Agricultural Research Center for Hokkaido Region (NARCH), National Agriculture and Food Research Organization (NARO)

• Sayama Mitsuru

  National Agricultural Research Center for Hokkaido Region (NARCH), National Agriculture and Food Research Organization (NARO)

• Sekiguchi Hiroyuki

  National Agricultural Research Center for Hokkaido Region (NARCH), National Agriculture and Food Research Organization (NARO)

• Ikeda Tsukasa

  Department of Applied Chemistry, Utsunomiya University

• Ishida Shigeki

  National Agricultural Research Center for Hokkaido Region (NARCH), National Agriculture and Food Research Organization (NARO)

抄録

Four hundred and fifty nine isolates of fluorescent pseudomonads were obtained from the leaves and roots of potato plants. Of these, 20 leaf isolates and 28 root isolates induced violacein production in two N-acylhomoserine lactone (AHL)-reporter strains—Chromobacterium violaceum CV026 and VIR24. VIR24 is a new reporter strain for long N-acyl-chain-homoserine lactones, which can not be detected by CV026. Thin-layer chromatography revealed that the isolates produced multiple AHL molecules. We compared the 16S rRNA gene sequences of these isolates with sequences from a known database, and examined phylogenetic relationships. The AHL-producing isolates generally separated into three groups. Group I was mostly composed of leaf isolates, and group III, root isolates. Group II comprised both leaf and root isolates. There was a correlation between the phylogenetic cluster and the AHL molecules produced and some phenotypic characteristics. Our study confirmed that AHL-producing fluorescent pseudomonads could be distinguished in the phyllosphere and rhizosphere of potato plants.<br>

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 24 (4), 305-314, 2009

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• fluorescent pseudomonad
• quorum sensing
• <i>N</i>-acylhomoserine lactone
• <i>Solanum tuberosum</i> L.
• 16S rRNA gene

詳細情報

• CRID: 1390001204347229056
• NII論文ID: 10025921199
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me09155
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 10463097
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10463097
• Web Site: http://www.jstage.jst.go.jp/article/jsme2/24/4/24_ME09155/_pdf
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可