Factors Controlling Long-Term Survival and Growth of Naturalized Escherichia coli Populations in Temperate Field Soils
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- Ishii Satoshi
- Department of Soil, Water, and Climate, University of Minnesota
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- Yan Tao
- BioTechnology Institute, University of Minnesota
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- Vu Hung
- Department of Soil, Water, and Climate, University of Minnesota
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- Hansen Dennis L.
- Department of Biology, University of Minnesota-Duluth
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- Hicks Randall E.
- Department of Biology, University of Minnesota-Duluth
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- Sadowsky Michael J.
- Department of Soil, Water, and Climate, University of Minnesota BioTechnology Institute, University of Minnesota
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While Escherichia coli is widely used as an indicator of fecal contamination of waterways, recent studies suggest that this bacterium may become "naturalized" to soils. In this study, we investigated the survival and growth of naturalized E. coli in temperate soil in northern Minnesota. A spontaneously-occurring, antibiotic resistant E. coli strain, KS7-NR, was added to a field site at 103 cells (g soil)-1. The survival and growth of E. coli KS7-NR were followed from June to October, by using colony counts on agar plates supplemented with antibiotics, and by using quantitative PCR (qPCR) with strain-specific primers developed based on suppressive subtractive hybridization. Both plate count and qPCR analyses indicated that E. coli KS7-NR survived for more than 2 months in the field soil. Laboratory experiments showed that soil temperature, moisture, nutrients, and the presence of other soil organisms influenced growth and survival of E. coli KS7-NR in soil. Moreover, our results indicated that summer drought conditions that occurred during the field study likely limited in situ growth of E. coli at the field site. Taken together, these results suggest that E. coli strains can survive long-term and grow in soils, provided that abiotic factors are within their tolerance limits.<br>
収録刊行物
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- Microbes and environments
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Microbes and environments 25 (1), 8-14, 2010
日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001204345587712
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- NII論文ID
- 10025921453
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- NII書誌ID
- AA11551577
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- ISSN
- 13474405
- 13426311
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- NDL書誌ID
- 10583309
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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