Development of a method for the determination of γ-aminobutyric acid in foodstuffs

• ISHIKAWA Akiko

  Department of Nutritional Science, Okayama Prefectural University

• OKA Hiroyuki

  Okayama Research Laboratory, Nihon-shokusei Co. Ltd.

• HIEMORI Miki

  Department of Nutritional Science, Okayama Prefectural University

• YAMASHITA Hiromi

  Department of Nutritional Science, Okayama Prefectural University

• KIMOTO Masumi

  Department of Nutritional Science, Okayama Prefectural University

• KAWASAKI Hitoshi

  Okayama Research Laboratory, Nihon-shokusei Co. Ltd.

• TSUJI Hideaki

  Department of Nutritional Science, Okayama Prefectural University

書誌事項

タイトル別名

• Development of a Method for the Determination of .GAMMA.-Aminobutyric Acid in Foodstuffs
• Development of a method for the determination of g aminobutyric acid in foodstuffs

抄録

A method for the determination of γ-aminobutyric acid (GABA) in foodstuffs has been developed by combination of its dinitrophenylation and high-performance liquid chromatography (HPLC) using norleucine as an internal standard. GABA was converted to its stable derivative with 1-fluoro-2,4-dinitrobenzene and the derivative was extracted with ether. After evaporation of the extract, the residue was dissolved in 0.1 M NaOH and the solution was subjected to reversed-phase HPLC with an elution system of a linear gradient of methanol in 10 mM Tris-HCl buffer (pH 6.0) and a detection system monitoring the absorbance of the effluent at 400 nm. The present method was shown to be utilized as a satisfactory method for the determination of γ-aminobutyric acid in foodstuffs.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｎｕｔｒｉｔｉｏｎａｌ　Ｓｃｉｅｎｃｅ　ａｎｄ　Ｖｉｔａｍｉｎｏｌｏｇｙ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｎｕｔｒｉｔｉｏｎａｌ　Ｓｃｉｅｎｃｅ　ａｎｄ　Ｖｉｔａｍｉｎｏｌｏｇｙ 55 (3), 292-295, 2009

  一般財団法人 学会誌刊行センター

キーワード

• γ-aminobutyric acid
• HPLC
• DNP-derivative
• determination of γ-aminobutyric acid

詳細情報

• CRID: 1390001206326565888
• NII論文ID: 10025971529
• NII書誌ID: AA00703822
• DOI: 10.3177/jnsv.55.292
• COI: 1:CAS:528:DC%2BD1MXnvF2mtbg%3D
• ISSN: 18817742
• ISSN: 03014800
• NDL書誌ID: 10252116
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10252116
• 本文言語コード: en
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