Characterization of a Cellular Immunostimulating Peptide from a Soybean Protein Fraction Digested with Peptidase R

• EGUSA Shintaro

  Interdisciplinary Graduate School of Science and Technology, Shinshu University Marusan-ai Co., Ltd.

• OTANI Hajime

  Interdisciplinary Graduate School of Science and Technology, Shinshu University

抄録

An immunostimulating glutamine-rich peptide was purified from a soybean protein fraction digested with Peptidase R produced by Rhizopus oryzae (Ro-digest) by a combination of SP-Sepharose column chromatography and reversed-phase high-performance liquid chromatography. The purified peptide was supposed to be located at or near the glutamine-rich region 202 to 222 of the glycinin G4 subunit. The peptide significantly increased the number of CD8⁺, CD11b⁺, and CD49b⁺ cells in C3H/HeN mouse spleen cell cultures, while 2 chemically synthesized glutamine-rich peptides corresponding to residues 202 to 213 (QQQQQQKSHGGR) and residues 214 to 225 (KQGQHQQEEEEE) of the glycinin G4 subunit increased the number of interleukin (IL)-12⁺CD11b⁺ cells. The peptide 202-213 also significantly increased the number of CD49b⁺, IL-2⁺CD4⁺, and interferon-γ⁺CD4⁺ cells and stimulated the cytotoxic activity of spleen cells toward the human erythroleukemia cell line K562. These results indicate that the glutamine-rich region of the soybean glycinin G4 subunit stimulates the cellular immune system in mouse spleen cell cultures.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｎｕｔｒｉｔｉｏｎａｌ　Ｓｃｉｅｎｃｅ　ａｎｄ　Ｖｉｔａｍｉｎｏｌｏｇｙ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｎｕｔｒｉｔｉｏｎａｌ　Ｓｃｉｅｎｃｅ　ａｎｄ　Ｖｉｔａｍｉｎｏｌｏｇｙ 55 (5), 428-433, 2009

  一般財団法人 学会誌刊行センター

キーワード

• glycinin G4
• Peptidase R
• cellular immunostimulation
• glutamine-rich peptide
• cytotoxic activity

詳細情報

• CRID: 1390282681302682496
• NII論文ID: 10025972201
• NII書誌ID: AA00703822
• DOI: 10.3177/jnsv.55.428
• COI: 1:CAS:528:DC%2BD1MXhtlOqur7F
• ISSN: 18817742
• ISSN: 03014800
• HANDLE: 10091/10765
• NDL書誌ID: 10401525
• PubMed: 19926930
• Web Site: https://soar-ir.repo.nii.ac.jp/records/10351
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10401525
• Web Site: https://search.jamas.or.jp/link/ui/2010097703
• 本文言語コード: en
• データソース種別:
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  • IRDB
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
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