Immunocytochemical Demonstration of the Synovial Membrane in Experimentally Induced Arthritis of the Rat Temporomandibular Joint.

DOI PubMed 被引用文献3件 参考文献33件
  • 井上 佳世子
    新潟大学歯学部口腔解剖学第二講座 2nd Department of Oral Anatomy, Niigata University School of Dentistry
  • TAKAGI Ritsuo
    2nd Department of Oral amd Maxillofacial Surgery, Niigata University School of Dentistry
  • KOBAYASHI Tatsuaki
    2nd Department of Oral amd Maxillofacial Surgery, Niigata University School of Dentistry
  • OHASHI Yasushi
    2nd Department of Oral amd Maxillofacial Surgery, Niigata University School of Dentistry
  • MAEDA Takeyasu
    2nd Department of Oral Anatomy, Niigata University School of Dentistry

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The present study is first to report an experimental model of adjuvant-induced arthritis in the rat temporomandibular joint (TMJ). Arthritis was induced by simultaneous intradermal administrations of Freund's complete adjuvant, one at the parietal scalp and the other at the base of the tail. In this model, we demonstrated responses of the synovial membrane by immunocytochemistry using antibodies to OX6 and ED1 which recognize Ia antigen in MHC class II antigen-expressing cells and the macrophage/monocyte lineage, respectively. Three weeks after administration, no remarkable signs of inflammation were macroscopically recognizable in the TMJ, but microscopically the synovial membrane in the TMJ revealed marked changes such as enhanced vascularization and hemostasis in the sublining layer and a thickening in the synovial lining cell layer. Intense OX6-immunoreactivity was found in the synovial lining cells at lesions in the experimental group but not in the control group. Immunoelectron microscopy revealed that these OX6-immunopositive synovial lining cells developed dense cytoplasmic processes and numerous vacuoles and vesicles, resembling type A cells. Part of the type A cells also showed ED1-immunoreactivity. The expression of OX6 or ED1 immunoreactivity in the synovial lining cells might be involved in the initial immune responses in this arthritis model because the synovial membranes are exposed to the synovial fluids which have been believed to contain antigenic substances.

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