Direct Plant Regeneration via Multiple Shoot Induction in Stylosanthes seabrana

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著者

    • KUMAR Suresh
    • Division of Crop Improvement, Indian Grassland and Fodder Research Institute
    • CHANDRA Amaresh
    • Division of Crop Improvement, Indian Grassland and Fodder Research Institute

抄録

We report an efficient protocol for <i>in vitro</i> direct plant regeneration <i>via</i> multiple shoot induction in <i>Stylosanthes seabrana</i>. <i>Stylosanthes</i> is an important range legume for humid to semi-arid tropics. It is very good fodder for animals, containing about 15% crude protein with 61% <i>in vitro</i> dry matter digestibility. <i>S. seabrana</i>, in particular, is rich in lysine and sulphur-containing amino acids, which are limiting amino acids in ruminants' feed. Limited genetic variability in the germplasm and susceptibility to anthracnose disease are major challenges in <i>Stylosanthes</i> improvement. <i>S. seabrana</i>, being a self-pollinated and diploid species, has been identified as a candidate species for improvement through genetic transformation. <i>In vitro</i> plant regeneration is one of the pre-requisites for development of transgenic <i>Stylosanthes</i> with desirable traits. In the present study, we used apical meristematic axis from <i>in vitro</i> grown seedlings as explant for multiple shoot induction. MS medium supplemented with BAP, Kn and TDZ, in different concentrations and combinations, were used for apical meristem culture. Best response, in terms of the number of shoots (4) per apical meristem, was observed on MS medium supplemented with 3.0 mg/l BAP. Shoot elongation was achieved on MS medium containing 2.0 mg/l GA and best rooting was induced on MS medium supplemented with 2.0 mg/l IAA. Tissue cultured plants showed normal growth, flowering and seed setting. To the best of our knowledge, this is the first report on plant regeneration via multiple shoot induction in <i>S. seabrana</i>. A standardized protocol would be very useful for <i>Agrobacterium</i>-mediated genetic transformation of <i>Stylosanthes</i> with the gene(s) of interest.

収録刊行物

  • Cytologia : international journal of cytology

    Cytologia : international journal of cytology 74(4), 391-399, 2009-12-25

    Japan Mendel Society, International Society of Cytology

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各種コード

  • NII論文ID(NAID)
    10026186122
  • NII書誌ID(NCID)
    AA00622764
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00114545
  • DOI
    10.1508/cytologia.74.391
  • データ提供元
    CJP書誌  J-STAGE 
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