Effect of essential oil of Anthemis mauritiana Maire & Sennen flowers on intestinal smooth muscle contractility

• Karim Ahmed

  Laboratoire de Physiologie et d'Ethnopharmacologie, Faculté des Sciences, Université Mohammed Premier

• Berrabah Mohammed

  Laboratoire d'Activation Moléculaire, Faculté des Sciences, Université Mohammed Premier

• Mekhfi Hassane

  Laboratoire de Physiologie et d'Ethnopharmacologie, Faculté des Sciences, Université Mohammed Premier

• Ziyyat Abderrahim

  Laboratoire de Physiologie et d'Ethnopharmacologie, Faculté des Sciences, Université Mohammed Premier

• Legssyer Abdelkhaleq

  Laboratoire de Physiologie et d'Ethnopharmacologie, Faculté des Sciences, Université Mohammed Premier

• Bouali Abderrahime

  Laboratoire de Génétique et Biotechnologies, Faculté des Sciences, Université Mohammed Premier

• Haloui Benyounes

  Laboratoire de Physiologie et d'Ethnopharmacologie, Faculté des Sciences, Université Mohammed Premier

• Amrani Souliman

  Laboratoire de Biochimie, Faculté des Sciences, Université Mohammed Premier

• Aziz Mohammed

  Laboratoire de Physiologie et d'Ethnopharmacologie, Faculté des Sciences, Université Mohammed Premier

抄録

The aim of the present study is to determine the chemical composition of the essential oil extracted from the flowers of Anthemis mauritiana Maire & Sennen (EOAM) and to investigate its antispasmodic effects on intestinal smooth muscle. The phytochemical composition was revealed by gas chromatography-mass spectrometer. Eighteen compounds were identified representing 90.56% of the oil. The major constituents were described as α-pinene (27.02%), sabinene (15.25%), cedrenol (14.53%) germacrene (9.61%) geraniol formate (6.82%), and caryophylene (5.38%). EOAM (10-100 μg/ml) elicited reversible relaxation of spontaneous contractions of isolated rabbit jejunal smooth muscle preparations, and similarly inhibited contractions induced by high-potassium solution ([K⁺]_o = 76 mM) and carbachol (10^-6 M) with IC₅₀ values of 14.98 and 27.29 μg/ml, respectively. Furthermore, EOAM exhibited an inhibitory effect on the dose-response curves induced by carbachol and CaCl₂ on rat jejunum preparations. These results clearly demonstrated the antispasmodic effect of EOAM which was strongly suggested to be mainly due to an inhibitory effect on Ca²⁺ influx through the membrane of jejunal smooth muscle cells.<br>

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｓｍｏｏｔｈ　Ｍｕｓｃｌｅ　Ｒｅｓｅａｒｃｈ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｓｍｏｏｔｈ　Ｍｕｓｃｌｅ　Ｒｅｓｅａｒｃｈ 46 (1), 65-75, 2010

  日本平滑筋学会

キーワード

• essential oil
• <i>Anthemis mauritiana</i> Maire & Sennen
• jejunum
• antispasmodic

詳細情報

• CRID: 1390282680032777088
• NII論文ID: 10026187234
• NII書誌ID: AN10364204
• DOI: 10.1540/jsmr.46.65
• ISSN: 18848796
• ISSN: 09168737
• Web Site: http://www.jstage.jst.go.jp/article/jsmr/46/1/46_1_65/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010265276
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可