Developmental Ability of Miniature Pig Embryos Cloned with Mesenchymal Stem Cells

• LEE Sung-Lim

  College of Veterinary Medicine, Gyeongsang National University Research Institute of Life Sciences, Gyeongsang National University

• KANG Eun-Ju

  College of Veterinary Medicine, Gyeongsang National University

• MAENG Geun-Ho

  College of Veterinary Medicine, Gyeongsang National University

• KIM Min-Jung

  College of Veterinary Medicine, Gyeongsang National University

• PARK Jun-Kyu

  Gyeongnam Livestock Promotion Research Institute

• KIM Tae-Suk

  Gyeongnam Livestock Promotion Research Institute

• HYUN Sang-Hwan

  College of Veterinary Medicine, Chungbuk National University

• LEE Eun-Song

  College of Veterinary Medicine, Kangwon National University

• RHO Gyu-Jin

  College of Veterinary Medicine, Gyeongsang National University Research Institute of Life Sciences, Gyeongsang National University

抄録

The present study compared the developmental ability of miniature pig embryos cloned with fetal fibroblasts (FFs), bone marrow-derived mesenchymal stem cells (MSCs) and differentiated (osteocytes, adipocytes and chondrocytes) MSCs. MSCs were isolated from an approximately 1-month-old female miniature pig (T-type, PWG Micro-pig^®, PWG Genetics Korea). MSCs were differentiated into osteocytes, adipocytes and chondrocytes under controlled conditions and characterized by cell surface antigen profile using specific markers. These differentiated or undifferentiated MSCs, as well as FFs of miniature pig, were transferred into enucleated oocytes of domestic pigs. Data from 10 replicates involving 1567 cloned embryos were assessed in terms of developmental rates. The in vitro development rate to the blastocyst stage of embryos cloned with undifferentiated MSCs was significantly (P<0.05) higher than that of embryos cloned with differentiated MSCs and FFs. Surgical transfer of 523 two-cell stage embryos cloned with undifferentiated MSCs into five synchronized domestic pig recipients resulted in 5 cloned miniature pig offspring (1 stillborn and 4 viable) from 2 pregnant recipients. The results imply that MSCs might be multipotent and that they can be used to produce viable cloned miniature pigs that cannot be easily reproduced with differentiated somatic cells.<br>

収録刊行物

• Journal of Reproduction and Development

  Journal of Reproduction and Development 56 (2), 256-262, 2010

  公益社団法人 日本繁殖生物学会

キーワード

• Animal cloning
• Differentiation
• Mesenchymal stem cells
• Miniature pig
• Nuclear transfer

詳細情報

• CRID: 1390282681314790144
• NII論文ID: 10026324600
• NII書誌ID: AA10936678
• DOI: 10.1262/jrd.09-196a
• ISSN: 13484400
• ISSN: 09168818
• NDL書誌ID: 10656561
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10656561
• Web Site: http://www.jstage.jst.go.jp/article/jrd/56/2/56_09-196A/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2010255741
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可