Molecular Identification of Genotypes of Pilophorus typicus (Heteroptera: Miridae) in Japan using PCR-RFLP Analysis of Mitochondrial DNA
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- ITO Katsura
- JST Innovation Satellite Kochi Faculty of Agriculture, Kochi University
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- NISHIKAWA Hiroshi
- Faculty of Agriculture, Kochi University
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- SHIMADA Takuji
- Faculty of Agriculture, Kochi University
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- OGAWA Kohei
- Faculty of Agriculture, Kochi University
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- MINAMIYA Yukio
- Faculty of Agriculture, Kochi University
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- HAYAKAWA Hiroshi
- Faculty of Agriculture, Kochi University
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- FUKUDA Tatsuya
- JST Innovation Satellite Kochi Faculty of Agriculture, Kochi University
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- ARAKAWA Ryo
- JST Innovation Satellite Kochi Faculty of Agriculture, Kochi University
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Pilophorus typicus (Heteroptera: Miridae) is a candidate insect for biological control against agricultural pests such as aphids, whiteflies, and spider mites. This species has two genotypes in Japan, i.e., the northern and southern types, but it is difficult to differentiate them because of few morphological characters separating them. Artificial migration across the distribution range of each genotype may incur ecological and genetic risks. To conduct a prompt identification of the two genotypes, we developed the method of PCR-RFLP using the cytochrome oxidase subunit I (COI) region on mitochondrial DNA. The PCR product of the southern type yielded two DNA bands by the action of the restriction enzyme of Psi I, whereas that of the northern type gave only a single band on electrophoresis. Moreover, the PCR-RFLP using samples from many localities revealed that the restriction site of Psi I is well conserved within each type. These results suggest that the PCR-RFLP of the COI region provides the accurate and easy discrimination of the two genotypes of P. typicus.
収録刊行物
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- Environment Control in Biology
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Environment Control in Biology 47 (4), 191-195, 2009
日本生物環境工学会
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詳細情報 詳細情報について
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- CRID
- 1390282680237223936
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- NII論文ID
- 10026429646
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- NII書誌ID
- AA12029220
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- COI
- 1:CAS:528:DC%2BC3cXisFerurw%3D
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- ISSN
- 18830986
- 1880554X
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- NDL書誌ID
- 10531894
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- CiNii Articles
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- 使用不可